Question about molecular biology

Assignment Help Biology
Reference no: EM1386472

Assume you infected an E. coli culture with a virulent bacteriophage. Most of the cells lysed, but a few survived - 1x10^-4. You wonder where the resistant bacteria came from. Were they caused by the bacteriophage infection or did they already exist in the bacterial culture? Earlier, for a different experiment, you ha spread a dilute suspension of E. coli onto solid medium in a large Petri dish, and, after seeing that about 10^5 colonies were growing up, you made an imprint of the colonies on that plate and transferred it to three other plates - a process known as replica plating - which creates identical patterns of the colonies on 3 plates. You realize that you can use these plates to distinguish between the two possibilities. You pipette a suspension of the bacteriophage onto each of the 3 replica plates so that the bacteria can be infected. What result do you expect if the bacteriophage cause resistance? What result do you expect if the resistant bacteria pre-exist?

Reference no: EM1386472

Questions Cloud

How many cars would you expect to see in the system : How many cars would you expect to see in the system. A toll tunnel has decided to experiment with the use of a debit card for the collection of tolls. Initially, only one lane will be used
What torque is required to the motor : A 24.00 kg child is on a swing that hangs from 1.6-m long chains. What is her highest speed if she swings out to a 45° angle.
Find running time of heap sort input sorted-ascending order : Determine the running time of Heap Sort if input is sorted in ascending order. Determine the running time of Heap Sort if input is sorted in descending order.
Formulate a spreadsheet model that will give the profit : Formulate a spreadsheet model that will give the profit in part b for any of the two numbers. Use the Excel goal seek command to calculate the break-even point found in part a.
Question about molecular biology : Assume you infected an E. coli culture with a virulent bacteriophage. Most of the cells lysed, but a few survived - 1x10^-4. You wonder where the resistant bacteria came from.
Determine the amount by which the spring is stretched : A 9.10 kg board is wedged into a corner and held by a spring at a 50° angle, as the Illustrating shows. The spring has a spring constant of 196 N/m and is parallel to the floor.
Determine the mode shapes and frequencies of vibration : Determine the mode shapes and frequencies of vibration of the system of cars by Sweeping the rigid body mode
The objective is to find a production plan that maximizes : The objective is to find a production plan that maximizes the daily profit. Formulate your model mathematically and solve it by using the Excel solve
Define the average force exerted on the ball : While a certain rubber band is stretched a distance x, it exerts a restoring force of magnitude F=ax+bx^2, where a and b are constants. The work completed in stretching this rubber band from x=0 to x=L is what.

Reviews

Write a Review

 

Biology Questions & Answers

  Synaptic signalling between adjacent neurons

A rabbit taken from a meadow near sea level and moved to a meadow high on a mountainside would have some trouble breathing. Why give an appropriate reason.

  Analyze the scientific literature

The flagellum is quite literally an outboard motor that few bacteria use to swim. It is rotary device that, like a motorboat, turns propeller to push against liquid, moving bacterium forward in process.

  Surgical procedure requiring general anesthesia

A patient was hospitalized for a surgical process requiring general anesthesia. After surgery, it was noted that there were few to no bowel sounds and her bladder was not emptying as it should.

  Cells of salmonella typhimurium

Assume you ingest 20 cells of  Salmonella typhimurium  in a chicken taco. Some of the bacteria are killed as they pass through the stomach but ten viable cells survive and reach the intestine.

  Compute the changes in na+

Compute the changes in Na+ due to an action potential. In other words, by what factor does the [Na+]I change because of an action potential firing?

  Frogs are extremely sensitive to acid rain

Frogs are extremely sensitive to acid rain. How might this information be used to investigate your observation about the trees dying in the forest.

  Question about biometry statistics

In 1997, the magazine Public Interest published an article entitled IQ and economic success  in which can be discovered the given table relating IQ to earnings

  What is the exact benefit relationship

Recent evidence shows that the individual chromosomes occupy fairly defined territories within the nucleus. Given the structure and location of parts of the nucleus, which would be more probably involved in chromosome location.

  What is the biochemical pathway

What is the biochemical pathway that corresponds to duplicate dominant epistasis in genetics? There is a 15:1 ratio of phenotypes, there are two genes involved: A and B. but what is biochemical pathway that causes this ratio to form.

  Design two degenerate primer sequences

Develop two degenerate primer sequences that will amplify the entire Open reading frame of the corresponding gene in a PCR reaction.

  Find out the genotypes of the two black females

Find out the genotypes of the two black females and the brown male using allele symbols B and b. One shows linear carbon chains, another has one -CH3 groups present on the third carbon atom, and third has two -CH3 groups present on the second carbon ..

  The f1 generation consisted of wild-type males and females

In Drosophila a cross was made between a yellow-bodied male with vestigial (not fully developed) wings and a wild-type female (brown body). The F1 generation consisted of wild-type males and wild-type females.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd