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Question: A breaking buffer contains 10mM Tris, pH 8.0, 150mM Nacl. The elution buffer is breaking buffer that also contains 300mM imidazole. Describe how the instructor made the 0.5 L elution buffer for students using a 1M stock of Tris (121.1 g/mole)(pH8.0), 5M Nacl (MW= 54.88g/mole) and 1Mimidazole (68.08g/mole).
An individual heterozygous for 4-gene pairs AaBbCcDd was testcrossed to aabbccdd , and progeny were classified as follows,
State the probability (percentage or fraction) of each of following sets of parents generating the given genotypes within their offspring.
Draw a drop of blood from the aorta to the skin of the face and back to the right atrium. Comprise, in order, the main arteries and veins.
Which lower vascular plant group once had tree-sized individuals (ca. 250- 300 mya) in swampy areas; their fossilized remains became a significant part of most coal deposits in the US.
Roger has arthritis and has been taking prednisone (a glucocorticold) at prescribed levels for two months. He isn't feeling well and complains of repeated colds. He is puffy (edematous). Describe the reason for these symptoms.
A pregnant woman is worried about the chances of her child having hemophilia as both she and her husband have paternal grandfathers with hemophilia.What are the likely genotypes of the couple
Compare changes in carbon cycle to that of the nitrogen cycle on human health. Create a conceptual graphical model for how changing the carbon cycle is likely to impact human health.
suppose a bird that eats grasshoppers moves into the prairie. How will this affect natural selection of the grasshoppers? How might this change in a drought year.
While Mexican hairless dogs are crossed with normally haired dogs, about half the pups are hairless and half have hair. While, though, two Mexican hairless dogs are mated, about a third of the pups produced hair, about two thirds are hairless and som..
Determine which of the following can contribute to stabilization of the secondary structure of a protein? (Select ALL that are TRUE.
Assume you wish to conduct a chi-square test on a set of data obtained from a test cross, to determine whether two genes (A and B) are linked.
In addition to protein phosphorylation and dephosphorylation, protein degradation also plays key role in regulating signal transduction pathways and cell proliferation.express how protein degradation affects cell proliferation.
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