Prepare standard curve by mixing monosaccharide dilutions

Reference no: EM1393405

Assume you perform a colorimetric enzyme assay to determine the activity of invertase in a bioreactor (total volume = 1L) used to produce inverted sugar. You prepare your standard curve by mixing known monosaccharide dilutions (3mL) with the DNS reagent (2mL). The standard curve follows the following linear function:

Absorbance (540nm) = 1.82 X monosaccharride concentration (M)

You mix 1.5 mL of the enzyme solution from the bioreactor with 1.5 mL of substrate solution and let them react for exactly 10min, after which you stop the reaction by adding 2mL of DNS reagent. You then add 20mL of distilled water and read the absorbance value at 540nm.

You obtain an absorbance value of 0.395.

Determine the reaction rate (mol monosaccharide formed / min) in the bioreactor.

Reference no: EM1393405

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