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A common practice in early stages of research is to simulate the conditions of blood using a simple buffered system. You are tasked with the responsibility to prepare 250 mL of buffer with a total concentration on 125 millimolar(mM) at a pH of 6.85 as a matrix representing blood suffering from a slight case of acidosis.
(a) Of the three options given below, select the best acid as a starting point for the preparation of this buffer in one sentence explianing your reasoning a (HOCH2)3CH3Cl 8.51 x 10-9 CH3CO2H 1.74 x 10-5 NaH2PO4 6.31 x 10-8
(b) Calculate the mass (in milligrams) of the orginal solid you selected in part (a) and the volume of a solution of 6.0 M NaOH (in mL) that would be requiored to prepare the desired buffer?
(c) give one specirfic potential source of error that could have occured during the preparation of the buffer and explian in detail how you would expect it to affect either the final pH or overall concentration of the buffer?
Use the Henderson-Hasselbalch equation to evaluate the pH of a buffer solution that is 0.25 M in formic acid (HCO2H) and 0.55 M in sodium formats (HCO2Na).
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