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The PCR-derived DNA fragment used an insert in pUC19 was amplified by 25 cycles.The amplification mixture contained, among other components, 1 ng lambda DNA in 50 uL. If you assume that the PCR amplification process and restriction digestion process are 100% efficient, how many pmol of product (1110 bp fragment) were produced?How mnay ug of DNA (1110 bp product) were present after amplification.The 1110 fragment was digested with BamHI and HindIII to form the 784 bp product. How many ug of DNA (the 784 bp product) were formed? Average MW of 1 bp= 660 g/mol
Explain physiological changes that occur in three different organ systems when food is ingested and how these systems coordinate a response in the human body.
In a co-evolved predator-prey relationship, does predation rate operate under directional selective pressure or stabilizing selective pressure?
Animals obtain energy throughout a series of chemicals reactions in which sugar (C6 H12 O6) and oxygen gas (O2) are reactant. This produces water (H2O) and carbon dioxide (CO2) as waste products. How might you use a radioactive isotope to find out wh..
Discuss the most likely complication of any placental fragments are retained within the uterus? Define subinvolution.
Suppose when a virus goes out of lysogeny to finish the lytic pathway, it loops up. DRAW this looping just prior to recombining out of the chromosome.
The trend in the U.S. is having children later in life than previous generations. What effects may this trend have on the incidence of late-acting dominant lethal alleles in the population.
There were many extinction events since life began. Permian extinction is a very important event. Explain why was it so important?
List two types of macromolecules that have been partly digested by the time acid chyme moves into the intestine. Where did this digestion take place and what enzymes were involved.
Assume you are studying metabolic pathways and discover that 2-pathways intersect so that enzyme basinase participates in both of the intersecting pathways.
Develop a CLADOGRAM showing how human, kangaroo, duck, chicken and Neurospora are related to each other.
define how prokaryotes and eukaryotes provide different. What does each category tell you about evolution; pick one from an early era and one from today or recent times.
Assume you are analyzing microbial life in the Rocky Mountains. You scoop up a water sample from a melted snow puddle because you are curious about the possible microbes that might live there.
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