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Antibodies: A. Consist of 2 heavy chains & 2 light chains held together by disulfide bonds. B. Can be used to detect proteins associated with their subcellular structures in living cells by indirect immunofluorescence microscopy. C. Cannot be used to affinity purify proteins because antigen-antibody complexes cannot be dissociated without denaturing the antigen. D. Cannot recognize an epitope as small as a single phosphorylated amino acid. E. (a) (b) (c) and (d) Indirect immunofluorescence microscopy utilizes: A. A fluorescently tagged primary antibody that binds by its Fc region to an epitope on its corresponding antigen. B. A fluorescently tagged primary antibody that binds by its CDR to an epitope on its corresponding antigen. C. A fluorescently tagged secondary antibody that binds by its CDR to the Fc region of a primary antibody bound by its CDR to an epitope on its corresponding antigen. D. A fluorescently tagged secondary antibody that binds by its Fc region to the CDR of a primary antibody bound by its Fc region to an epitope on its corresponding antigen. E. A GFP-tagged protein that is directly visualized in a living cell. How would you examine the localization of a chimeric GFP-fusion protein in a living cell using an epifluorescence microscope? The λexcite for GFP is 395 nm & λemission is 509 nm. A. Just view the cell at 395 nm as GFP is self-fluorescing. B. Just view the cell at 509 nm as GFP is self-fluorescing. C. Irradiate or illuminate the cell at 395 nm & view it at 509 nm. D. Irradiate or illuminate the cell at 509 nm & view it at 395 nm. The masses (kDa = kilodaltons) & protein:lipid ratios of three human plasma lipid transport particles are: VLDL (very low density lipoprotein particle) ~10 kDa; 1:100 LDL (low density lipoprotein particle) ~2 kDa; 25:100 HDL (high density lipoprotein particle) ~0.2 kDa; 90:100 Based on this information, which two methods could be effectively used in combination to separate these three different lipid transport particles from each other? A. Equilibrium density-gradient sedimentation and LC/MS-MS B. Equilibrium density-gradient sedimentation and ion exchange chromatography C. Gel filtration chromatography and differential centrifugation D. Differential centrifugation and equilibrium density-gradient sedimentation. E. Equilibrium density-gradient sedimentation and gel filtration chromatography
What is the probability that his son would develop
The Twenty mL of water is added to the Erlenmeyer flask is to previously boiled, deionized water. Since water does absorb CO2 from atmosphere and since CO2 dissolved in water causes it to be slightly acidic,
If you cross a black fur rabbit and a white fur rabbit, the resulting offspring look gray, when examining closely the fur of gray rabbit consist of both black and white hairs. What type of non-mendelian inheritance is this.
Horn Sporophytes are horn-shaped. Horn Possess Gemmae. Can undergo sexual reproduction. Mosses what is the synapomorphy of hornworts, mosses and vascular plants? Stomata Pores that allow the uptake of CO2 and release of O2, do not close like other st..
What could you do presynaptically to change presynaptic repsonseand change the postsynaptic response to a second stimulu
Which kinds of spores are produced from a Peyote plant?
for transformation of Synechocystis, why do you first plate your transformant mixture on a filter on a BG - 11 plate with glucose, and the next day the TA transfers the filter with cells to a plate that contains kanamycin (rather than plating dire..
A male fruit fly with red eyes and long wings is mated to a female with brown eyes and short wings. The F1 offspring consist of red eyed and long-winged females, red-eyed, shortwinged males.
Cells have several mechanisms to prevent the production and accumulation of truncated protein fragments. To illustrate why these fragments can be deleterious.
What is the name of the vein that delivers the water soluble monosaccharides from the small intestine to the liver, the organ that gets "first dibs" on the glucose?
Diagram and explain the function(s) of the following biological molecules that comprise the plasma (cell) membrane: phospholipid bilayer, membrane proteins, carbohydrates.
What are the chances that a family with three children will have three daughters? After a family has two daughters, what is the probability that their next child will be a daughter?
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