Increasing magnification from high dry to oil immersion

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Question: You have 2 ml of a 1 mg/ml IgG solution. How would you prepare a series of dilutions with the following IgG concentrations: 0.4 mg/ml, 0.2 mg/ml, 0.1 mg/ml, 0.05 mg/ml. You will need at least 1 ml of each of these four concentrations.

Question: Suppose when increasing magnification from high dry to oil immersion, should the iris diaphragm be open or closed? How is this done? Does this adjustment increase or decrease the light reaching the objective?

Reference no: EM1392004

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Increasing magnification from high dry to oil immersion : Suppose when increasing magnification from high dry to oil immersion, should the iris diaphragm be open or closed? How is this done?
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