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In terms of the structural aspects of hb that we discussed

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In terms of the structural aspects of Hb that we discussed in class, explain asmany features of the following Hb variants as you can (you may not be able toexplain all of the properties).

Sketch an O2 binding curve for each variant,comparing it to Mb and HbA curves. The columns, O2 affinity, Bohr effect, Hillcoef. (coefficient) and BPG effect, refer to that property of the variant comparedto normal HbA at neutral pH with 0.1 M NaCl ("normal" conditions).

Also,indicate how the affected subunit would change the electrophoretic mobility ofthe Hb tetramer in non-denaturing native electrophoresis.

a. Variant: Hb Hiroshimaß146 His->Asp

Bohr effect: reducedO2 affinity: increasedHill coef: 2.0BPG effect: decreased

b. Variant:Hb Bassetta94 Asp->Ala

O2 affinity: reducedBohr effect: reducedHill coef: 1.4BPG effect: normal

Reference no: EM13585443

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