Reference no: EM133926418
Microscopy Worksheet
1. How many oculars does your microscope have? What is the magnification of your ocular(s)?
2. When you move the slide slowly to the right. In what direction does the image in the ocular move? Is this the same or different than what the slide is doing?
3. What are two ways you can adjust the amount of light on your specimen?
4. How do you focus your microscope? Why is it important to focus in low power and avoid using the course adjustment at higher power?
5. What is the total magnification of a specimen when viewing the specimen with the 40x objective? Show your calculation
6. Can you see the same amount of your ruler at high power as you did at low power? What is the relationship between field size and magnification?
7. Based on your measurements, what is your field size at 4x? Use that information to calculate the field size at 40x. Show your calculations.
Define:
- Isotonic
- Define hypertonic
- Define hypotonic
- Define plasmolysis
8. What happened to the Elodea cells when you added distilled water to the slide vs. the 10% NaCl?
9. What happened to the Elodea cells when you added tank water back to the slide? Based on some research, is plasmolysis reversible? Why was/wasn't it in your observations? Why or why not?
10. What are tracheids? What role to they play in water transport in xylem? Draw a picture of the tracheids (celery string) you observed.
11. Do some research and provide a reason why some plants may have more stomata than others and what would cause them to open or close their stomata. Provide your reference(s).
12. Describe double fertilization in angiosperms.