Find the titer of b-d-galactosidase

Reference no: EM1339160

The purpose of the experiment that took place was to determine the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay. I used a formula obtained from class mates to find the titer. I want to be sure this is the correct method for determining the titer. I attached my data, including the results from the plate reader, and my graphs of the log of the inverse of the antibody dilution vs. absorbance. The formula I used to find the titer of rows C and D are below. If this is not the correct method for finding the titer, what is, and how is it performed? And what do the results of the titers mean?

Row C
y = -0.033x + 0.1864

A450 max = 0.263
½ max = 0.1315
0.1315 = -0.033x + 0.1864
-0.0549 = -0.033x
1.6636 = x
10^(1.6636) = 46.09
Titer = 46.09

Row D
y = -0.0415x + 0.2306

A450 max = 0.339
½ max = 0.1695
0.1695 = -0.0415x + 0.2306
-0.0611 = -0.0415x
1.4723 = x
10^(1.4723) = 29.67
Titer = 29.67

Reference no: EM1339160

Questions Cloud

Explain employment law- layoffs and ageism : Explain Employment Law- Layoffs and Ageism and The employees vary in age and length of service, with half of the employees being above the age of 40 and half being below the age of 40

How to define the terms anabolic steroid : how to Define the terms anabolic steroid. Discuss the potential risks and dangers associated with using steroids and human growth hormone.

What do you think marketing ethics is central : Are marketing ethics critical to successful marketing. What do you think marketing ethics is central to building brand loyalty.

Introduction of gunpowder weapons : How did the introduction of gunpowder weapons change the social, political, and economic contexts of warfare in Europe?

Find the titer of b-d-galactosidase : find the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay.

Elucidate how would the different forces come together : Elucidate how would the different forces come together to create a convergence between the interests of stockholders and managers.

Explaining schedule variance and cost performance index : What is the cost variance, schedule variance, cost performance index (CPI), and schedule performance index (SPI) for the project?

Comparing peace of god and the truce of god : Comparisons are made for these two subjects: Peace of God and the Truce of God.

Explain implications of certain employment laws : Explain Implications of Certain Employment Laws and If this restrictive covenant essentially means the employee would need to relocate or commute to work over one hour one-way every day to get to an employer that is far enough away not to violate t..

User Account

All Pages