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1. In this lab, you will add 4uL of 1M IPTG to 4mL of bacterial culture. What is the final concentration of IPTG in the bacterial culture?
4 uL = 0.004 mlMf = Mi x Vi / VfMf = 1 x 0.004 / 4 = 0.001 M
2. Predict how many bands of DNA you will see in the double digest ifa. you have GST only?One (circle)
b. you have GST-Cherry?Two
3. The teacher was not paying attention and put the red lead into the black slot and the black lead into the red slot of the power supply. This reverses the current as shown below. In this incorrect orientation, where will the DNA end up (at the top of the gel, at the bottom, stuck in the wells, dissolved, etc)?
Darwin understood how natural selection works in the presence of a population with many variant types. What does that variation consist of? How does variation arise? How does it spread during the population.
Why was the discovery of microsphere and coacervates an important contribution to understanding of how life might have originated on earth .
Suppose you are interested in studying dogs as a system for obesity. You crossed a Cocker Spaniel to a Labrador Retriever and interbred the offspring for many generations.
A child is suspected of having an eating disorder. What hormones require to be assessed prior to considering that the situation is primarily psychological?
One of the major categories of receptors in the plasma membrane reacts by forming dimmers, adding phosphate groups, and then activating relay proteins. Which kind does this.
define the relationship between chromatin packaging and transcription rates. Name two different types of chromatin modification associated with changes in gene expression.
Butterflies are typical diploid eukaryotes with the following inherited situations, True-breeding c s o are crossed to c+ s+ o+ to produce F1 progeny
If a man with a red-colorblindness (a sex-linked trait) marries a normal woman, would you expect to see trait expressed in their children.
Suppose a biallelic locus in a diploid population has the genotype frequencies: A1A1= 0.59; A1A2 = 0.16; and A2A2 = 0.25.
Bradford Assay was performed with samples in triplicate. I got the standard curve and using three abosorbance from the plate reader for the samples and then I extrapolated the protein samples.
When a tropical moth is touched on the thorax it lifts its forewings up abruptly, exposing its brightly coloured hind-wings. What causes the moth to behave this way? What significance can we attach to the fact that the verbal ability scores of ado..
Assume that in other ltxnsp cells the HCO 3 - in the ICF = 1mM, 10mM in the ECF, and is passively distributed across the membrane.
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