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While separating the components of a mixture of Blue Dextran, cytochrome c, as well as DNP-glycine by ion exchange chromatography, the eluent is changed from 0.1 M acetate buffer to 1.0 M acetate buffer once the first colored component begins to exit the column. Explain why is the eluent changed from low salt concentration to high salt concentration? Define the order in which the components would elute from the CM Sephadex column.
Which has the lowest freezing point: CaF2, (NH4)3PO4, C6H12O6, or NaNO3?
when chlorine gas is bubbled into a solution of a sodium bromide the sodium bromide reacts to give bromine a red- brown
What is the temperature of the sample, in degree celsius? can you please show steps?
How much heat is produced by the complete combustion of 256 CH4 of ? CH4+2O2=CO2+2H2O Hrxn=-802.3kJ
How density affect if some of the water in the graduated cylinder splashes out when the sample is dropped into the cylinder?
calculate the molarity of each of the following solutions: a) .500 mol of sucrose in .200 L of a sucrose solution. b) 30.4g of LiBr in 350 mL of a LiBr solution. c) 73.0 g of HCl in 2.00 L of a HCl solution.
The methyl benzoate and acetanilide react with HNO3, H2SO4, and glacial acetic acid. Need to know the possible products and then the one that actually forms.
You need to prepare 100.0 mL of a pH=4.00 buffer solution using 0.100 M benzoic acid (pKa = 4.20) and 0.160 M sodium benzoate. How much of each solution should be mixed to prepare this buffer
to determine the partial pressure and the total pressure using daltons law of partial pressures.a mixture of gases
What volume (mL) of a concentrated solution of magnesium chloride (9.00 M) must be diluted to 350. mL to make a 2.75 M solution of magnesium chloride
describe the soil and site characteristics of soilderived from older fresh water sediments as one of the six soil
Starting with 0.86 moles of HF, calculate the number of moles of H2O that is produced by this balanced reaction.
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