Explain what order will the aminoo acids elute

Assignment Help Chemistry
Reference no: EM13291446

A mixture of Phe (pI 5.5), Thr (pI 6.5), Asp (pI 3.0), His (pI 7.6), and Lys (pI 9.8) at pH 9.8 is loaded on an anion-exchange column and then eluted with a pH gradient of lowering pH. what order will the aminoo acids elute? Explain.

Reference no: EM13291446

Questions Cloud

Draw and label the equivalent circuit for motor conditions : Consider a DC series motordesigned to operate with VS = 200 V. Suppose the rotorresistance is 0.40 ohms. and the field coil resistance is0.10 ohms . An RPM measurement reveals that when the line currentis 35 A when the motor is spinning at n = 500..
Explain ph would recommend to separate cytochrome : At what optimum pH would you recommend to separate cytochrome C; pI 10.6 from a mixture od myoglobin; pI 7.0, ribonuclease; pI 7.8, and leucine; pI 6.0 using a Dowex 50 column? Explain.
An individuas personality is the characteristics : An individual's personality is the characteristics and qualities they display that can influence their behavior. Personality can affect the level of energy and the direction that energy is focused towards
Determine the power factor of the induction motor : An induction motor has thefollowing specifications: a 8-pole squirrel cage rotor, operates at25 hp off of a 220 V line-to-line 3-phase service, 60 Hz, 64 ampsof line current, and under full load runs at 830 RPM. The motor draws 20.8 kW at full loa..
Explain what order will the aminoo acids elute : a mixture of Phe (pI 5.5), Thr (pI 6.5), Asp (pI 3.0), His (pI 7.6), and Lys (pI 9.8) at pH 9.8 is loaded on an anion-exchange column and then eluted with a pH gradient of lowering pH. what order will the aminoo acids elute? Explain.
Design an inverting amplifier using an operational amplifier : Design an inverting amplifier using an operational amplifier such that the the voltage gain is 100. Write the formula for the output voltage. Design an adder using an operationalamplifier such that the output is the weighted sum of three inputs ba..
Using a specific commercial and referring to ideas : Reflection paper around 500 words long. Imagine you are a different sex (or gender). Would watching television commercials be different now for you? Explain why, using a specific commercial and referring to ideas discussed in the instructor’s notes a..
What role did the mass media play in the rally effect : What is the "Rally" effect? What are the essential elements needed to produce the Rally effect? How were these conditions met or not met in Haiti and Panama? What are the limitations on the Rally effect? How are they relevant in analyzing Panama/Hait..
Find the increase in temperature of the nail : A carpenter is driving a nail into a board. The 1.25 kg hammer moves at 8.85 m/s when it strikes the nail. find the increase in temperature of the nail after 3 blows from the hammer

Reviews

Write a Review

Chemistry Questions & Answers

  Determine the values of delta e

Determine after how many days will one-sixteenth of a given amount of radon-222 remain and Determine the values of Delta E and Delta H for the system?

  Compute the final temperature of the mixture

Calculate the final temperature of the mixture, assuming no heat is lost. The heat capacities of Hg and H2O are 27.98 and 75.33 J/mol/K, respectively.

  Define the van der waals equation of state

Derive an expression for (δU/δV)T for a gas that obeys the van der Waals equation of state.

  Calculate the number of kilograms of helium needed

Calculate the number of kilograms of helium needed to inflate a balloon to a volume of 100,000 L at an atmospheric pressure of 250 mmHg and a temperature of -35 °C.

  Compute how many such photons are emitted

A 60.0-W incandescent light-bulb produces about 4.00% of its energy as visible light. Assuming that the light has an average wavelength of 510.0 nm, calculate how many such photons are emitted per second by a 60.0-W incandescent lightbulb

  State the production of fluorine-containing compounds

Carbonyl fluoride, COF2, is an important intermediate used in the production of fluorine-containing compounds. For instance, it is used to make the refrigerant carbon tetrafluoride, CF4 via the reaction

  Explain the buret is filled with the naoh titrant

The buret is filled with the NaOH titrant and the initial volume reading is immediately recorded without waiting the recommended 10-15 seconds. However, in part b-1, the 10-15 second time lapse does occur before the reading is made.

  How many grams of water could be vaporized

giving that water has a molar enthlphy of vaporization of 40.79 kJ/mol, how many grams of water could be vaporized by 623 J

  State what is the rate law

What is the rate law for step 2 of this reaction? Express your answer in standard MasteringChemistry notation. For example, if the rate law is type k*[A]*[C]^3.

  Which solution would increase the osmotic pressure of water

Which solution would increase the osmotic pressure of water the most?

  Explain the initial rate of a reaction was measured

The initial rate of a reaction 2A + 3B -> C + 2D was measured using different initial concentrations of A and B. Use the information in the table to determine the rate law for the reaction.

  What is the aluminium concentration

A stock solution is prepared by adding 20 mL of 1.21 M AlCl3 to enough water to make 72 mL. What is the Al3+ concentration of 30 mL of the stock solution

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd