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Aliquots of a 0.5-mg/mL standard of BSA are utilized to construct a standard curve for the Bradford protein assay. The tubes contain the subsequent amounts of the BSA solution: 0, 20, 40, 60, 80, and 100 μL. The corresponding absorbencies subsequent to adding Bradford reagent are 0, 0.05, 0.09, 0.14, 0.19 and 0.22. If you take 20 μL of an unknown as well as add 80 μL of water, mix, take 10 μL of the mixture and add to Bradford reagent as well as see an absorbance of 0.08, Explain what is the protein concentration of the undiluted unknown?
Neutron stars are believed to be composed of solid nuclear matter, primarily neutrons.If the radius of a neutron is 1.0 × 10-13 cm, calculate the density of a neutron in g/cm3. (volume of a sphere = ) Assuming that a neutron star has the same dens..
Calculate delta G at 298 K for the formation of 1 mole of 03 from 02 in urban smog, where [O2]=0.210 atm and[O3]=5.00x10^-7 atm
Calculate the pH during the titration of 20.00 mL of 0.1000 M HCOOH(aq) with 0.1000 M NaOH(aq) after 20.22 mL of the base have been added. Ka of formic acid = 1.8 x 10-4.
Calculate the standard free energy change for the process N2O5(s)-->N2O5(g) at 25 0C if Vapor pressure of N2O5 at 7.5 degrees C is 100mmHg, and the solid sublimes at a pressure of 1.00 atm at 32.4 0C.
Discuss C is Trans nomenclature as it is utilized to distinguished among isomers with that of the E/Z system of nomenclature. Name the subsequent compound using the appropriate system.
One of the treatment for gangrene and "the bends" is a hyperbaric oxygenation. this occurs in a chamber in which oxygen is used at higher than normal pressures.
Predict the physical state of ammonia at -95 degrees C (mp =-77degrees C, Bp =-33 degrees C) and normal atmospheric pressure.
Draw the skeletal structure for trans-1-bromo-2-isopropylcyclopentane. (Hint: Make sure the tapered end, i.e. the thin end, of the wedge bond is attached to the parent.)
Predict the balanced chemical equation, including the correct state of matter (gas, liquid, aqueous, solid), for the reaction of iron nitrate with potassium oxalate.
Propanoic acid has Ka of 1.4x10^-5. If we make a solution 0.100M propanoic acid, what concentration of sodium propanoate should be added to give a buffer with a pH of 5.0?
When 35.6 L ammonia and 40.5 L oxygen gas react at STP, NO(g) and water are produced. How many grams of NO(g) are produced assuming 100% yield?
Lake Lianna is fed by two rivers, the Matthew and the Andrew. You have made careful water chemistry measurements throughout the year and found that the two rivers have the following pH and alkalinity
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