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A 50 mL crude extract of skeletal muscle contains 32 mg of protein per mL. Ten microliter of the extract catalyzes a reaction at a rate of 0.14 micromol product formed per minute. The extract was frationated by ammonium sulfate precipitation, and the fraction precipitating between 20% and 40% saturation was redissolved in 10 mL. This soluction contains 50 mg/mL protein. Ten microliters of this purified faction catalyzes the reation at a rate of 0.65 micromol/min.
a) what is the degree of purification (fold purification)?
b) what is the percent yield of the enzyme recovered in the purified fraction?
A NaOH soln of unknown concentration was standardized against KHP. It took 45.78 mL of NaOH soln to completely titrate 1.256 g of KHP. Cal the molarity of NaOH.
Find deltaH for the combustion of ethanol (C2H6O) to carbon dioxide and liquid water from the following data. The heat capacity of the bomb calorimeter
How many total moles of ions are released when the following sample dissolves completely in water? 7.24x 10^20 formula units of NiCl2.
calculate the temperature (in EC) at which the vapor pressure is 760 torr. The vapor pressure of Ethanol is 115 torr at 34.9EC
determine if the brominated product (2,3-dibromo-3-phenylpropanoic acid) is contaminated with some unreacted starting material (during the bromination of trans-cinnamic acid)?
what fraction of this side chain is positively charged and what fraction is neutral at pH 7.2?
The empirical formula for acetic acid is CH2O. Its molar mass is 60 g/mol. what the molecular formula is?
3.8 g of a hydrocarbon fuel is burned in a calorimeter that contains 234 grams of water initially at 25.00 ? C. After the combustion, the temperature is 26.55 ? C
At what temperature would a 1.20 M NaCl solution freeze, given that the van't Hoff factor for NaCl is 1.9? K_f for water is 1.86 C/m
Determine how many grams of Hydrogen are required to form 14.01grams of Ammonia and calculate how many grams of ammonia could be formed
A graduated cylinder contains 133 of water. A 10.0 piece of iron (density = 7.86 ) and a 16.0 piece of lead are added. What is the new water level in the cylinder?
The solubility product for Pb3(PO4)2 is 1.0 x 10^-54. write out the solubility reaction equation explaining why it is set up that way using the solubility rules or else I will not really understand how to set up the problem.
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