Explain the preparation of tris buffer

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Tris is widely used in a biochemical laboratory. Its acid dissociation constant is 8.32 x 10^-9. You have available at your lab bench a 0.1 M solution of Tris in its protonated form, 0.1 M solution of HCL and NaOH, and ample distilled water. Describe the preparation of 1L solution of 0.02M Tris buffer, PH 7.8. Please don't just write numbers.. explain it.

Reference no: EM13148924

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