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Genomic DNA from the nematode worm Caenorhabditis elegans is organized by nucleosomes in the manner typical of eukaryotic genomes, with 145 bp encircling each nucleosome and approximately 55 bp in linker DNA. When C. elegans chromatin is carefully isolated, stripped of non-histone proteins, and placed in an appropriate buffer, the chromatin decondenses. Suppose researchers mix a sample of this chromatin with a large amount of DNase I that randomly cleaves DNA in regions that are not protected by bound proteins. Next, they remove the nucleosomes, separate the DNA fragments by gel electrophoresis, and stain the fragments with ethidium bromide.
a. Approximately what range of DNA fragment sizes do you expect to see in the stained electrophoresis gel? How many bands will be visible on the gel?
b. Explain the origin of DNA fragments seen in the gel.
The signaling mechanisms used by a steroid-hormone receptor and by an ion channel linked receptor have very few components. Can either mechanism lead to an amplification of the initial signal? If so, how? Ideas are included.describe an amplificati..
Determine the initial osmotic pressure at room temperature of a cell if the only ions present are CaCl2 on either side of the membrane. Suppose the concentrastions for K+ and Cl- from Table 7.2.
A women is perspiring profusely with rapid breathing, breath smells like acetone and blood sugar is 650mg/ml, blood chemistry indicates acidosis, what is her diagnosis, is it hypoglicemia, but thrown off by rapid breathing.
Which term describes tonicity of the external solution. Were latitude the only control of temperature, the isotherms would run straight across the map from east to west. Describe one region of the world where this hypothetical isotherm pattern is rea..
The formation of surface blebs caused by ischemia reflects in part plasma membrane injury, but also a sign of cytoplasmic injury.
Ed Rivers, a 3rd year medical student, was alone in the hospital ER one night. It was unusually quiet that night, and the resident was getting some much needed sleep.
As a biologist it is your job to look for plants that have evolved structures with a selective advantage in dry hot conditions. Which of the adaptions would be least likely to meet the objective.
State the key products of the Kreb's Cycle and how various of each there are starting from 1 glucose molecule.
An enzyme and its substrate are combined in a test tube but no product is formed. Another molecule is added to the tube, and now the product is formed at the normal rate. Give 2 reasons.what type of inhibition is working on the substrate.
Prader-Willi syndrome is caused by the deletion of the paternal copy of a small section of human chromosome fifteen. In normal individuals, only the paternal copies of the genes in this section of chromosome fifteen are expressed;
Utilize your imagination to describe two ways in which disturbance of the sort just described may affect the animals that live on the panels and amount of free space available.
A patient displays the enlargement of lateral and third ventricles, however no enlargement of fourth ventricle. Explain what do you conclude? At the time of a spinal tap of the patient, blood is discovered within the CSF. What does this finding pro..
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