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Here is the amino acid sequence of the enzyme (in single letter code):
MAAMDMDQFLAAAIDAAKKAGQIIRKGFYETKHVEHKGQVDLVTETDKGCEELVFNHLKQLFPNHKFIGEETTAAFGVTELTDEPTWIVDPLDGTTNFVHGFPFVCVSIGLTIGKVPVVGVVYNPIMEELFTGVQGKGAFLNGKRIKVSAQSELLTALLVTEAGTKRDKATLDDTTNRINSLLTKVRSLRMSGSCALDLCGVACGRVDIFYELGFGGPWDIAAGIVIVKEAGGLIFDPSGKDLDITSQRIAASNASLKELFAEAMMLTAA
Design two degenerate primer sequences that will amplify the entire Open reading frame (ORF) of the corresponding gene in a PCR reaction. To facilitate the cloning of the gene after PCR amplification, place a restriction endonuclease site at each end of your primers. You may choose any restriction endonuclease site you like, or use EcoRI (GAATTC). Hints: restriction endonuclease sites are cleaved more efficiently when 1-2 extra nucleotides are included at each end; these extra nucleotides are usually Gs or Cs. You need a forward and a reverse primer. In general, primers that are 18-30 nucleotides long are suitable for PCR amplification. Write out your primer sequences 5’ to 3’.
1. Forward primer sequence:
2. Reverse primer sequence:
What disorder does this man have? Gastric secretions normally include about 10mmole/L potassium. How do you account for the low serum potassium in this patient.
In this example, for every base designated Q, there is twice that amount of base R; for every base Z, there is twice that amount of base S. If molecule contains 33.33 percent R, what percentage of Z would you expect.
Use chi-square test, test whether these two loci show independent assortment? How would you explain the frequency of the progeny genotypes?
What function of intermediate filaments have in eukaryotic cells.define structure of microfilaments and microfilament network.
What do you think is the most probable mechanism of browning? If a patient's resting cardiac output is 5.6 l/min and on a stress test she elevated her heart rate to the highest of 176 beats/min with a stroke volume of 115 ml/beat, what is her cardiac..
In detail explain the structure and function of the heart and blood flow. Include the conduction system and blood vessels. Provide your response specific to your audience who are a group of lay people without a background in science.
When the sperm cell unites with egg cell this is known as ______. Usually, this happens in the ______. After four or five days, the developing zygote arrives the uterus.
What percentage of the father's body was covered by burns? What percentage of the daughter's body received first-degree burns?
Explain why is 5% (w/v) Sucrose solution hypotonic to the water plant cell, however a 5% (w/v) NaCI solution is hypertonic?
Two consecutive crosses, with the first between pure-breeding parents that differ in two phenotypes to create an F1 generation, and the second in two F1 progeny to create an F2 generation.
In mountain rabbits, the EL-1 gene is located on chromosome three. 7-alleles of this gene have been identified in the population. With respect to EL-1,
Biochemistry-michaelis menten eqn problem
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