Explain laemmli buffer to make the right concentration

Reference no: EM13156949

You have to run SDS-PAGE (a gel electrophoresis method for separating proteins with different MWs). You have to make the protein sample in a buffer called Laemmli buffer (LB) with DTT. The laboratory has a 5M concentration of Laemmli buffer and 1M DTT solution already ready for you. Your final protein sample should be in 1M concentration of LB buffer with 20mM DTT. Your gel sample loading capacity is 50 µL/well. Show how many µL of protein sample you will take and add with the desired volume of DTT and Laemmli buffer to make the right concentration of sample.

Reference no: EM13156949

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