Explain how to determine exclusion limit of a gel

Assignment Help Chemistry
Reference no: EM13169767

1. Explain 2 advantages of using Kd values instead of calculating the raw elution volume of your protein on your gel. 2. Explain how to determine exclusion limit of a gel of unknown origin.

 

Reference no: EM13169767

Questions Cloud

Explain what is the voltage of the cell : What is the voltage of the cell: silver-silver chloride electrode || saturated calomel electrode given that the potential for the Ag|AgCl electrode in a saturated KCl
Explain why is normalization of a probability distribution : Why is normalization of a probability distribution important? What would one have to consider when working with a probability
Compute the work function of tungsten : light of wavelength 221nm striking a sample of tungsten causes electrons to be ejected with a speed of 6.04x10^5 m/s. calculate the work function of tungsten
Explain examination of the product mixture revealed : Examination of the product mixture revealed that only about half of the starting material had been consumed and practically no PhCOCHBrCH3 was present. Explain these observations and draw the product that did form in the reaction.
Explain how to determine exclusion limit of a gel : Explain how to determine exclusion limit of a gel of unknown origin.
A very crude model of the buckminsterfullerene molecule : A very crude model of the buckminsterfullerene molecule (C60) is to treat it as a collection of electrons in a cube with sides of length equal to the mean diameter of the molecule (0.7nm)
State components of an acrylamide gel effects qualities : Which components of an acrylamide gel effects qualities of the final product, and which components affect the speed of polymerization?
Explain the purpose of running both dalton vll mixed markers : What is the purpose of running both Dalton Vll mixed markers and lanes with individual markers on SDS-PAGE?
Explain why is sodium bicarbonate used with acids : Why is sodium bicarbonate used with acids and used in cooking

Reviews

Write a Review

Chemistry Questions & Answers

  What mass of indium is present

A sample of indium sulfate, In2(SO4)3, contains 0.0446 moles of oxygen. What mass of indium is present?

  What is the total pressure in the container

The following reaction takes place in a sealed 40.0-L container at a temp of 120 degrees C 4NH 3(G) + 5 O2 (g) --> 4NO (g) + 6H2O (G)

  How much heat is gained (in joules) by the water

How much heat is gained (in Joules) by the water where a chemical reaction takes place in 100 mL aqueous solution and has a temperature increase of 14 C?

  What is the density of this compound

The compound ZnI4 is best described as anions in a face-centered cubic unit cell with the cations in the tetrahedral holes. What is the density of this compound?

  What volume would an equal mass of ethanol occupy

A mass of mercury occupies 0.750 L. What volume would an equal mass of ethanol occupy? The density of mercury is > and the density of ethanol is 0.789 g/mL.

  Determine adiabatic flame temperature of combution process

A fuel gas containing 22% CO, 13% CO and 65% N2 by volume is combusted with theoretic amount of air in a furnance to heat solid burden material.

  Explain barium sulfate is used in medical imaging

Barium sulfate, , is used in medical imaging of the gastrointestinal tract because it is opaque to X rays. A barium sulfate solution, sometimes called a cocktail

  What are the values of delta h and delta e

Assume that a particular reaction evolves 239 kJ of heat and that 27 kJ of PV work is gained by the system. what are the values of delta H and delta E? Assume that a particular reaction evolves 239 kJ of heat and that 27 kJ of PV work is gained..

  How many moles of khp were originally present

dissolved .31 grams of KHP in 25 ml of water and it required 15.5ml of NaOH to reach end of titration, how many moles of KHP were originally present before the addition of the NaOH?

  Calculate the percentage yield of magnesium chloride

for the reaction Mg+2HCl yeilds H2+MgCl2, calculate the percentage yield of magnesium chloride if 100.g of magnesium react with excess hydrochloric acid to yield 330.g of magnesium cholride.

  What is the composition of vapor of a pentane-hexane

what is the composition of vapor of a pentane-hexane solution that has a vapor pressure of 373 torr at 25 degree celcius? Given is Pure Hexanes and pentanes vapor pressure at 25 degrees celcius. Pentane - 511 torr. Hexane -150 torr.

  Combustion of one mole of glycerol

Calculate G°f (kJ mol -1) for glycerol and G°rxn (kJ mol -1) for the combustion of one mole of glycerol

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd