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Which of the following is the best reason as to why the strands of a DNA molecule are more easily separated at pH > 11?
A) At pH 11 the cross-strand negative charge repulsion becomes so strong that the two strands are much more easily separated.
B) Most biomolecules precipitate when the pH is greater than 11. Upon precipitation the two strands are much more easily separated.
C) Any solution above pH 11 is considered highly caustic and therefore many of the covalent bonds, especially between the nitrogenous bases and the C1' sugars, will be hydrolyzed at this extreme pH.
D) The pKa values for some of the hydrogen bond donor groups (e.g., R-H2) are lower than 11 and therefore these groups will become deprotonated at pH values above 11. The deprotonated form of these groups cannot form the favorable cross-strand hydrogen bonds that hold the two strands together.
Assume that sucrose is a molecular solid and does not ionize when it dissolves in water. Kf values are given here.
What is the amount of volume of the solvent you need to extract 65g of the solute in one extraction from the original 100g dissolved in 206mL water?
A container (8.65-L ) holds two gases mixture at 31 °C. The partial pressures of gas X and gas Y, respectively, are 0.206 atm and 0.867 atm. If 0.200 mol a third gas is added with no change in temperature or volume determine the total pressure be..
draw a qualitative potential energy diagram for the rotation about the c3-c4 bond in 2,4-dimethylpentane
write structural formulas for the following condensed structures and determine the functional group in each compound.
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estimate the mean ionic activity coefficient for NaCl in a solution that is 0.020 mol/kg NaCl(aq) and 0.035 mol/kg Ca(NO3)2 (aq)
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The liquid rate used is twice the minimum, the equilibrium relationship can be represented as yA=9.95 xA then determine what is HOG, operating line relationship, NOG and packed height of the column?
Calculate the change in entropy (in J/K) for the following reaction at 298 K: C(s) + H2O(g) ? CO(g) + H2(g) Use entropy for graphite (diamond is too expensive).
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