Explain apparent affinity constant for the competition assay

Reference no: EM13274969

Using the thermodynamic cycle in the tight binding KNI-764/HIV-1 protease example discussed in class, derive the Kapp= KKNI-764/(1+KAc-pepstatin[AcPepstatin]) for the apparent affinity constant (Kapp) for the competition assay between the weak (Ac peptstatin) and tight (KNI-764) inhibitor binding to HIV-1 protease. write expressions for HIV-1 protease complexed with each of the inhibitors individually, and remember that in the competition assay, only the unbound protein can bind KNI-764

Reference no: EM13274969

Questions Cloud

Calculate the value of a right : Image Storage Corporation has #1,000,000 shares outstanding. It wishes to issue 500,000 new shares using rights issue. If the current stock price is $50 and the subscription price is $47/share, calculate the value of a right? a. 0.40/right b. 5.00..

Explain the only two accessible conformations of a protein : The only two accessible conformations of a protein differ by 2kJ/mol. What percentage of protein molecules will be in the higher energy state at 350K? at 270K

What will be the carrier frequency variation corresponding v : A 5-volt intelligence amplitude causes the frequency to change to ± 25 kHz. What will be the carrier frequency variation corresponding to an intelligence amplitude of 10 volts

Design and simulate a power supply that takes line voltage : Design and simulate a power supply that takes line voltage(120v) and produces a 15V output with less than .5% ripple voltage with the following specifications use either full wave or full bridge wave.

Explain apparent affinity constant for the competition assay : Using the thermodynamic cycle in the tight binding KNI-764/HIV-1 protease example discussed in class, derive the Kapp= KKNI-764/(1+KAc-pepstatin[AcPepstatin]) for the apparent affinity constant (Kapp) for the competition assay between the weak (Ac..

Find the vswr coefficient of a 50 ohm transmission line : A 50 ohm transmission line is terminated to the load (60-40j) ohm. How far from the load, a short ckt stub of a fix length of λ/8 needs to be placed so the minimum voltage standing wave ratio (VSWR) between generator and stub achieved. Find the VS..

Describe two examples of vulnerabilities in automobiles : Describe two examples of vulnerabilities in automobiles

Discuss the fundamental advantages and disadvantages : Companies that currently use expert systems. Next, discuss the fundamental advantages and disadvantages of using expert systems instead of human expertise within the companies that you have selected. Provide a rationale to support your response..

Explain the empirical and molecular formulas of quinine : The molar mass of quinine is 324 g/mol. Determine the empirical and molecular formulas of quinine. (Type your answer using the format CO2 for CO2 and use the order CHNO) empirical molecular

User Account

All Pages