Evaluate the equilibrium constant for the reaction

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Reference no: EM13335302

The hydration of CO2, CO2 + H2O ? HCO3 - + H+, is catalyzed by the enzyme carbonic anhydrase. The steady-state kinetics of the forward (hydration) and reverse (dehydration) reactions at pH 7.1, 0.5°C, and 2 mM phosphate buffer was studied using bovine carbonic anhydrase. Some typical results for an enzyme concentration of 2.3 nM are Hydration Dehydration 1/v c(CO2) 1/v HCO3 - 36 · 103 1.25 95 · 103 2 20 · 103 2.5 45 · 103 5 12 · 103 5 29 · 103 10 6 · 103 20 24 · 103 15 Make suitable plots of data and determine the Michaelis constant KM and the rate constant k2 for the decomposition of the enzyme-substrate complex to form product for a. The hydration reaction. b. The dehydration reaction. c. From your results, calculate the equilibrium constant for the reaction CO2 + H2O ? HCO3 - + H+ (Hint: Start by writing an equation that defines the equilibrium condition in terms of the Michaelis-Menten formulation of this reaction. Note that the kinetics was measured at pH 7.1.)

Reference no: EM13335302

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