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1. During purification of many cytosolic proteins, it is important to include either dithiothreitol or b-mercaptoethanol in the buffers. Why?
2. What is the correct order of steps for muscle contraction
Why is blue-white screening important to biotechnology? Please be as specific as possible. We did an experiment transforming E. coli with plasmid DNA.
q1. in the hin recombinase system of salmonella typhimurium discussed in class what do you assume would be the effect
Discuss how does column chromatography separate molecules such as DNA and RNA? Explain whyis it difficult to separate large amounts of RNA and DNA using gel electrophoresis?
I need a drawing of a typical spherical virus which in addition to the standard structures, also has an envelope. It should be labeled three dimensions with parts removed to allow the viewer to see details inside the various layers. Thanks Will rate ..
What is the possibility that differences this great between observed and expected values would happen by chance. Two genes organize in corn snakes as follows: O-B is brown snakes, O-bb are orange, oo bb are albino. An orange snake was mated to a blac..
To determine which protein or proteins, A, B, C, or D, are responsible for the activation of DNA synthesis, you construct mutant versions of the PDGF receptor that retain one or more tyrosine phosphorylation sites.
With the completion of the human genome and the identification of various genes, it may be possible to determine whether an individual has a particular disease.
Calculate the physiological standard free energy change for the combined reactions in glycolysis converting 1,3-bisphosphoglycerate to pyruvate.
How are chromosomes rearranged in deficiencies, duplications, paracentric inversions, pericentric inversions, and reciprocal translocations?
A 41 year old homeless man with a history of alcohol abuse was brought to the ER by his friend. The patient had a productive cough which produced a-foul-smelling, purulent sputum. He had a fever and poor dental hygiene. Chest x-ray showed infiltra..
Describe the permeability properties of the axon membrane when the nerve is at rest and the permeability properties during the production of an action potential.
calculate the percent recovery for the analysis of a spike. the sample was spiked with 5.00 ml of a 200.0 mg/l solution. the sample matrix concentration was determined to be 30.0 mg/L. The sample matrix volume (excluding the spike) was 90.00 ml. T..
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