Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Question - An unknown protein (i.e., not BSA) binds to Carboxy Methyl (CM) -cellulose (pKa 4) at pH 7.0.
The loading buffer is 0.1M imidazole pH7.
a. Draw the two structural forms of the CM functional group. (Hint: see second page).
State the charge on the form which predominates at pH 7.0.
b. What is the charge on the protein at pH 7.0? Explain.
c. How can the bound protein be eluted from this particular resin?
Explain using 2 different methods describing the type of buffer used for each elution.
d. What can you conclude about the pI of the bound protein? Explain.
calculate the weight of sodium hypochlorite, NaOCl.
Imagine a person accidentally swallows a drop of liquid oxygen, which has a density of 1.149 g/mL. Assuming the liquid drop has a volume of .075mL, calculate the volume that would be produced when the liquid vaporizes in the person's stomach at 37..
An inclined manometer is being designed to measure pressure using mercury and water. Pressure differences as small as 100 Pa (0.001 atm) must be measured, but it is desired to have a minimum reading of 1.0 cm. At what angle from horizontal must th..
Explain why titanium is a good choice of material for golf clubs. When not in use, discuss at least one precaution to observe when storing clubs?.
Calculate the pH of the solution in a flask after 25 mL of NaOh has been added. Presume 0.413g of KPH is dissolved in 25mL of water and then titrated with 0.1M NaOH.
If 4.00 g of the unknown compound contained 0.133 mol of C and 0.267 mol of H, how many moles of oxygen, O, were in the sample?
A solution is prepared by dissolving 6.00 g of an unknown nonelectrolyte in enough water to make 1.00 L of solution. The osmotic pressure of this solution is 0.750 atm at 25oC. What is the molecular weight in g/mol of the unknown solute
A buffer that has .370 m base and .234 m of its conjugate acid contain ph of 9.09. what is the ph after .002 mol of HCL is added to .250 l of this solution.
How many moles of NaNO2 will be contained in the 1 liteer, 1.34 solution after it is made? b. What volume of concentrated 5.48 M NaNO2 solution will contain this number of moles?
Write your specific and critical viewpoint on Donald Marquis' theory that most abortion theories completely miss the point.
Could you explain the Kinetics of a Reaction Lab experiment where we determine the rate of the reaction between Crystal Violet dye and Sodium Hydroxide? I want to be able to understand how the how the whole reaction takes place
The Apparatus shown consists of three bulbs connected by stopcocks. What is the pressure inside the system when the stopcocks are opened?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd