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Discuss the theory behind the spectrophotometer

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Reference no: EM131706829

Introduction

Discuss the importance of protein determinations in the laboratory biochemistry setting.

Discuss Beer's Law in detail, and the appropriateness of this law for concentration measurements in biological samples.

Describe three different methods of protein determination and their advantages and disadvantages.

Discuss the theory behind the spectrophotometer

Methods

Describe the methodology described for protein determination via the Bradford assay.

Data

Plot the data that you collected and calculate the concentration of the unknowns.

Use linear regression analysis to analyze the data and calculate the protein concentrations from the line equation.

On the wavelength scans, identify the peaks that are caused by the absorption of light by the unbound and protein-bound Coomassie dye.

Discussion

Discuss the data analysis and method of unknown protein concentration calculations. Discuss the results from the scan of the unbound and protein-bound wavelength scans.

References

Information in your report should be referenced as full citations in the format style of your choice, APA as preference.

Attachment:- Assignment.rar

Reference no: EM131706829

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