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In a gene expression experiment for determining biochemical pathways in Serratia marcescens, are the mutant strains utilized in this experiment true auxotrophs? Why or why not?
Assume you have a gel filtration column with unknown matrix. Describe how do you experimentally deteremine its void volume and exclusion limit
Justify the hypothesis of early microorganisms were chemolithotroph and why phototrophic organisms that came after had pigments that absorbed maximally at UV and IR radiation
A plasmid digest with BamHI produced 2 kb and 4 kb fragments. Digest of a plasmid with HindIII produced 6 kb fragment. Double digest with both enzymes produced 1 kb, 2 kb and 3 kb fragments. Sketch a plasmid map.
Assume your professor handed you a test tube with 3.0 ml of an E.coli culture in it and told you to make a 10^(-3) dilution of the entire culture. Describe how you would do it.
A mixture of very hot and very cold water. Organize them, so your right hand is in the front of cold water, your left hand is in front of hot water, and lukewarm water is in the middle.
Ecoli with mutated Oric sequences can still replicate its chromosome, though very slowly. One mechanism of this origin independent replication depends on initiation of transcription.
What genotypic frequencies are expected at birth in the next generation. How much change has there been in the frequency of the B allele.
Which lower vascular plant group once had tree-sized individuals (ca. 250- 300 mya) in swampy areas; their fossilized remains became a significant part of most coal deposits in the US.
During sleep, relay neurons in the thalamus reduce information reaching the cerebrum by altering their membrane potential. In diagnosing bacterial and viral infections of the nervous system, samples of CSF are extracted from the spinal cord.
Assume you ask is to work with the genetic code and examine the structure and characteristics of the amino acids used to make proteins and answer the following question.
Explain why are estuaries, like the Pamlico Estuary, so significant? Summarize the two different camps of the thought on life cycle of Pfiesteria.
Discuss blood types, the factors in crime situations. Determine what is a criminal case where blood samples were important in the case decision and outcome, what was it about the blood in this situation?
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