Determine the vt and vo for the column

Reference no: EM13687186

Question- The figure in Appendix I gives an elution profile for the following proteins using Sephadex G-100 (Fractionation range 4KDa - 150 kDa):

0.9 KDa Amanitin

5 KDa Aprotinin

80 kDa Transferrin

135kDa Calmodulin

Part a) Give the Ve, in ml, for each protein.

Part b) Determine the VT and Vo for this column. If one or the other cannot be calculated, clearly state why.

Solve the above problem

Reference no: EM13687186

Questions Cloud

Draw the structures of salicylic acid and aspirin : Problem- Draw the structures of salicylic acid and aspirin. Circle and identify three functional groups present in each compound.

What is the concentration of ni2 ions at equilibrium : Problem- A 0.130-mole quantity of NiCl2 is added to a liter of 1.20 M NH3 solution. 1) What is the concentration of Ni2 ions at equilibrium. Assume the formation constant* of Ni(NH3)6^2+ is 5.5 × 10^8.

Explain the chlorine demand is 60% of the dosage : Problem- A 5-MGD surface water supply is treated with conventional filtration followed by chlorine disinfection. The chlorine dose is controlled to be less than 2 mg/L to avoid total trihalomethane (a disinfection by-product, or DBP) problems. The..

The average flow rate of a wastewater treatment plant : Question- The average flow rate of a wastewater treatment plant is 20 MGD and the peak factor is 2.0. Design the sedimentation tanks by using the following information

Determine the vt and vo for the column : Question- The figure in Appendix I gives an elution profile for the following proteins using Sephadex G-100 (Fractionation range 4KDa - 150 kDa)

What would be the specific rate constant for the reaction : Part 1) What would be the specific rate constant for the reaction. Part 2) if the initial concentration of A is changed to 2.oo molar and the initial concentration of B remains the same, what would be the initial rate of reaction

Calculate the change in ph when 6.00 ml of 0.100 m hcl : Calculate the change in pH when 6.00 mL of 0.100 M HCl(aq) is added to 100.0 mL of a buffer solution that is 0.100 M in NH3(aq) and 0.100 M in NH4Cl(aq).

Calculate the percent by mass of the solute propylene : Question- Calculate the percent by mass of the solute propylene glycol in a solution generated by mixing 1.46 kg of C3H8O2 with 7120 mL of the solvent water.

What volume (l) of the solvent tetrahydrofuran : Question- What volume (L) of the solvent tetrahydrofuran should be added to 0.358 L of the solute diethyl ether to prepare a solution that contains 7.60 % by mass (C2H5)2O.

User Account

All Pages