Determine proton motive force across mitochondrial membrane

Reference no: EM1394522

A. Consider a mitochondrion with a pH in the matrix = 8 and the pH of the intermembrane space = 5.9. The matrix is 100 mV more negative than the IMS or cytoplasm. What is the proton motive force (?p) across the mitochondrial membrane?
B. If the ratio of ATP to ADP and Pi is such that ?G for ATP hydrolysis = -60 kJ/mol, how many mols of H+ will have to move through the ATP synthase in order to generate a mol of ATP? [The answer should be whole number.]

Reference no: EM1394522

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