Determine km for glucose oxidase

Reference no: EM13169688

The enzyme glucose oxidase converts glucose to glucolactone and generates one equivalent of hydrogen peroxide. The cofactor flavin adenine dinucleotide (FAD) is required for function. The nucleoside adenine monophosphate is an uncompetitive inhibitor that binds to the FAD binding site with an association constant of Ka = 4x106 M-1. Glucose oxidase is observed to have a maximal velocity of 1.5 mmol/min for the oxidation of glucose. At a glucose concentration of 1x10-5 M the rate is 0.5 mmol/min. A. Determine KM for glucose oxidase. KM = M. B. Determine effect on the maximal rate if the concentration of adenine is 0.001 M. Vmax,I = mmol/min.

Reference no: EM13169688

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