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4. A company has designed a new weight loss drug. They want to determine if the drug is carcinogenic, so they use it in the Ames test. Bacteria used in the Ames test are His-auxotrophs, and all of the bacteria are plated on minimal media without histidine. The results are
- Test substance + rat liver + distilled water= 500- Test substance + distilled water=4- Distilled water=8- Rat liver enzymes=1- Positive control that has a known mutagen=675
The test substance is:A) mutagenB) a mutegen, but only when metabolizedC) not a mutagenD) can't tell - there is contamination in the experiment
5. Which of the following would you use to find a telomeric sequence?A) genomic DNA libraryB) cDNA libraryC) mRNA libraryD) Answers B and CE) All of the above
6. What is genomic DNA libraryA) A collection of vectors, each containing a fragment of the original genomeB) A complete sequence of the genetic material in an organismC) An electronic file that may be used for additional genetic analysisD) A collection of mRNA from a given cellE) None of the answers are correct
A 2.5 mg/ml stock solution was diluted as described in the table below and then the absorbance of each dilution was estimated and presented in the table given below.
There are a number of natural types of DNA. One is animal DNA, one plant DNA, another bacterial DNA.What are these two kinds of DNA.
What is a main property of DNase that makes it useful for assessing whether chromatin is in a closed (tightly condensed) or open (loosely packed) configuration.
In Leghorn chickens colored feathers are produced by a dominant allele, C; white feathers are produced by the recessive allele, c. The dominant allele, I, of another independent gene inhibits expression of color in birds with geno-types C/C or C/c.
Assume you have a gel filtration column with unknown matrix. Describe how do you experimentally deteremine its void volume and exclusion limit
The two biochemical pathways for coat color pigment in a species of triblles are shown below. The final color of the tribble is the combination of colors produced by the two pathways.
Illustrate the texture and arrangement of the lungs of the cat. If you were asked to create a karyotype from the chromosome spreads you made from HeLa cells, list materials you could use and describe how you would create the karyotype.
Examine the challenges faced by the Public Health System. Epidemiology and Evolving Health Care System
he clinical laboratory returns the following arterial blood values for patient: pH = 7.2; plasma HCO3- = 29 mmol/L; plasma pCO2 = 75 mmHg. Determine the patient's acid-base disorder?
While discussing their findings, authors have ignored a main threat to the validity of their estimates of the exposure. Explain and discuss this error.
Four proteins, each having a single polypeptide, are separated by SDS-PAGE: human cytochrome c , human serum albumin, bovine myoglobin.
An oligomeric protein has a molecular weight of 153000. A 960 mg sample was treated with an excess of 1-fluoro-2,4-dinitrobenzene(sanger's reagent) until the reaction was complete.
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