Describe sds-page, The given statements describe SDS-PAGE,

Describe sds-page

Reference no: EM1381772

The following statements describe SDS-PAGE, a method used to separate proteins. Some of the statements are correct, and some of them are incorrect. Select all the correct statements.

Statements:

-SDS-PAGE uses a polyacrylamide gel to separate proteins.

-Sodium dodecyl sulfate denatures the proteins, resulting in protein separation by charge only.

-Smaller proteins migrate farther than larger proteins

-In SDS-PAGE, the more negatively charged proteins elute first from the column.

-Sodium dodecyl sulfate dentures proteins, resulting in proteins that are the same shape. (Assume beta-mercaptoethanol is also present to reduce disulfide bonds.)

-In SDS-PAGE, proteins migrate toward the negative electrode.

Reference no: EM1381772

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