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Describe a method of sequencing that is based on synthesis i.e. "sequencing-by synthesis" that does not require the separation of newly sythesized DNA/RNA strands.
Compare it with the Sanger dideoxynucleotide method?
Assume you learned in class that DeltaG for compete oxidation of glucose is -686kcal/mole. According to the DeltaG for ATP and the numbers of ATP,
The relative humidity in a closed room with a volume of 645.2 m^3 is 87.6% at 300K, and vapor pressure of water at 300K is 0.0313 atm.
The red kernel color in wheat is caused by the presence of at least one dominant allele from each of the two idependently segregating gene pairs.
In examining a large sample of yeast colonies on a petri dish, a geneticist finds an abnormal-looking colony that is very small. This small colony was crossed with wild type, and products of meiosis (ascospores) were spread on a plate to produce c..
Calculate the generation time when an exponentially growing cell population increases from 5 x 10^6 cells/ml to 5 x 10^8 cells/ml in 8h. Calculate the number of cells/ml in a sample after counting a total of 143 cells in 25 squares of a counting c..
What is the probability that a daughter will be a carrier? Be prepared to draw a Punnet square on the board.
Describe the anatomy (structure) and physiology (function) of a plant. Is the structure of your plant a modification from the standard root, stem, leaf, or flower?
Define biodiversity and explain how it is most commonly measured. Now consider the various ways to define a species (i.e., species concepts) covered in the readings and explain how current estimates of biodiversity might change depending on the speci..
In cattle, coats may be solid white, solid black, or white and black spotted. When true breeding solid whites are crossed to true-breeding solid blacks, the F1 generation consists of all spotted individuals.
Describe a suitable control you could use for testing the specificity of the Bradford assay for specific amino acids.
How is palmitate made without ATP? I need the entire cycle shown or described in detail. I know that malonyl CoA which is required for palmitate synthesis in fatty acid metabolism uses ATP, so how do we bypass this?
Explain how Mark Keating found the sequence of the human HERG cDNA sequence. Make sure to explain how cDNA libraries were constructed and screened to find the unique clone - Explain how the inward rectifying potassium channel cDNA sequence was fou..
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