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Scientists are working on purifying PFK-1C from rabbit brain tissue. The rabbit brain tissue was homogenized and centrifuged to remove insoluble material. Next, the soluble preparation was loaded on top of an ATP-Sepharose column. This is an affinity column in which ATP is covalently linked to a polysaccharide bead. This sample is loaded on top of the column, washed with a low-salt buffer, followed by a wash with a high salt buffer. What is the rationale for this procedure and what would the expected elution profile look like? (Keep in mind PFK-1A Mr = 84000 dal, PFK-1B Mr = 80000dal, PFK-1C Mr=86000 dal)
What is the pH of a solution made by adding 8.80 grams of Sodium Acetate to 450mL of .105 M hydrochloric acid?(Assume no change in volume takes place)
Provide structures consistent with the following molecular formulae and spectral data, and assign spectral peaks. Write your structures and tables on a sheet of paper.
To resolve an object in an electron microscope, the wavelength of the electrons must be close to the diameter of the object. What kinetic energy must the electrons have in order to resolve a protein molecule that is 7.00 nm in diameter? Take the mass..
calculate the solubility of zinc hydroxide znoh2 in 1.00 m naoh.nbsp ksp 3.0 times 10-16 for znoh2 kf 3.0 times 1015
A diamond is made of pure carbon. The distance between any two neighboring carbon atoms in a diamond is 1.54 angstroms. What is the distance in inches
The half-life of C-14 is 5730 years. A bone sample with 3.6 g of carbon has 32.6 disintegrations per minute. How old is the bone sample in years?
if the reaction between iron iii ion and thiocyanate ion yielded an equilibrium concentration of .15m for each of these
61.0 ml of anbsp solution1.60 m is diluted to a volume of 208 ml. anbsp portion of that solution104-ml is diluted using
A 25.0-mL aliquot of vinegar was diluted to 250 mL on a volumetric flask. Titration of 50.0-mL aliquots of the diluted solution required an average of 34.88 mL of 0.09600 M NaOH. Express the acidity of the vinegar in terms of the percentage (w/v) ..
When a sample of helium gas is charged with electricity, what causes the bright-line spectrum that is observed
Calculate: Solution Density Mass fraction and % (sodium acetate) Mole fraction (sodium acetate) Molarity molality molality of solute particles
When 50.0 g of iron(III) oxide reacts with carbon monoxide, 32.8 g of iron is produced. What is the percent yield of the reaction? Fe2O3(s)+3CO(g)-->2Fe(s)+3CO2(g)
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