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1. You have a 2 kb plasmid, and you want to insert a gene thatgives ampicillin resistance that is 500 bp in size. You also wantto insert a gene for penicillin resistance that is 435 bp in sizeand your PCR product, which is 250 bp in size. What would the totalsize of the plasmid be following the insertions?
2. Why would a different column, one unlike the silica gel purification column used in the protocol, cause anunsuccessful transformation?
3. What would happen if you forgot to heat shock the E. coli cells during the transformation reaction?
What do you think could be happening: for the crows; for the virus? Provide a set of postulates, guided by evolutionary explanations.
Draw the peptide ATLDAK at pH7. What is its net charge? What is its approximate pI?
The ability to curl your tongue up on the sides (T, tongue rolling) is dominat to not being able to roll your tongue. A woman who can roll her tongue marries a man who cannot. Their first child has his father's phenotype.
Two indpendently assorted genes control the color and taste of blueberries. Expression of the dominant allele B results in blue color while expression of the recessive allele b results in white color.
You have a 1mM stock solution of Arsenic to treat culture cells in a liquid medium. You need to treat your cells with Arsenic such that it has a final concentration of 5nM once it has been added to the cells.
The nematocyst is a morphologically and functionally complex structure. Through what process are nematocysts formed by the cnidoblasts?
Write a hypothesis and null hypothesis
The new form of life is discovered. It has a genetic code much like that of organisms on Earth except that there are five different DNA bases instead of four and the base sequences is translated as doublets instead of triplets. How various different ..
What are the steps involved in forming the Ig mRNA(s) that will encode a complete immunoglobulin molecule? Include all steps at both the DNA and RNA level and the molecules involved in the process as well as their function.
Where are lysosomes formed?
Chickens have ZZ-ZW sex determination. The bar-feathered phenotype in chickens results form a Z-linked allele that is dominant over the allele for nonbar feathers. If a barred female is crossed with a non-barred male, what will be the phenotypes o..
How is it that different cells can respond in different ways to exactly the same signaling molecule even when they have identical receptors?
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