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The transformation efficiency is defined as the quotient of the nubmer of cells transformed and the amount of input DNA in μg. In a transformation experiment, 50μl of competent cells were mixed with a 100ng og plasmid DNA. After heat-shocking in the cell, you add 450μl of media, incubate for 1 hour and spread 70 μl of the transformation reaction on to several selective plates. the following day, you examine the plates and count an average of 200 colonies. Calculate the average transformation efficiency of this.
Describe how the evolution of roots and simple vascular transport expanded the habitat of plants onto land.
A father of a certain family belongs to blood group AB, and the mother to blood group O. They have four children, one belonging to blood group AB, one to group A, one to B and one to O.
Which process in eukaryotic cells will proceed whether O2 is present or not?
how to keeping the shape of the cell. But I have found that there is a primary and secondary cell wall - each has their own function - so I am somewhat confused and looking for guidance.
What are the roles of kinases in cell regulation? Why do you think kinases are used, and not another type of enzyme?
Chelsea, age 14, recently had a colectomy. Her colon was lined with thousands of polyps. Pathology reported that these polyps were adenomas and a diagnosis of familial adenomatous polyposis (FAP) was made.
Suppose if a prothallus is seperated in distance from other gametophytes, it will eventually produce both archegonia and antheridia.
Throughout pairing homologous regions associate with one another. As a result individual heterozygous for an inversion will form a structure known as an inversion loop. What is consequence of a crossover within this inverted region.
Throughout much of Europe, there has been a severe decline in mushroom population. On study plots in Holland, data collected since 1912 indicate that the number of mushroom species has dropped from 37 to twelve per plot in recent years.
Determine which of the below are types of analyses that can occur once an entire sequence of a genome is known?
Explain how cell division and the sexual reproduction help in genetic variability of organism. How do both the meiosis and sexual reproduction (fertilization) generate offspring which differ genetically from parents?
Albinism is a rare genetically inherited trait that is only in phenotype of homozygous recessive individuals. The most characteristic symptom is a marked deficiency in the skin and hair pigment melanin.
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