### Define the concentration of hemoglobin in the solution

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##### Reference no: EM13515129

A.) A UV/V is spectrometer is used to measure the absorbance of a solution of hemoglobin bound to oxygen at 590 nm. The molar absorptivity was determined to be 8.57 x 10^4 M^-1cm^-1. The cuvette used had a 1.00 cm sample pathlength. The measured absorbance was 0.180. What was the concentration of hemoglobin in the solution?

B.) What would the absorbance of the previous solution be if the pathlength were 1.80 cm?

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