Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Question: Ligation of the multiple ? fragments and pBLU produces a number of different hybrid molecules, although not as many as you might expect. Each clonable fragment must have one BamHI and one HindIII end, to combine with the BamHI and HindIII ends of the pBLU backbone and form a circular recombinant plasmid. Only 5 of the 13 BamHI/HindIII framgnets from the linear ? genome satisfy this requirement: 493 bp, 784 bp, 2,396 bp, 2,409 bp, and 4,148 bp. A sixth clonable fragment of 9,866 bp is derived from the COS circle. Multiple copies of a single fragment or combinations of two different ? fragments can also be ligated into a single pBLU backbone. Three fragment plasmids can be constructed by combining the pBLU backbone with one of hte five fragments having one BamHI end and one HindIII end and with one of the eight fragments having two BamHI or two HindIII ends. Constructs with more than one unit of pBLU are also formed. Oly constructs with at least one unit of pBLU backbone contain an origin necessary for replication and the ampicillin-resistance gene required for growth on selective media.
1. Make scale drawings of all potential two-fragment (vector + insert) recombinant plasmids. Include the total bp size, fragment bp sizes, and the locations of BamHI sites and HindIII sites, the origin, the amplicillin-resistance gene, and the lacZ gene.
Think about the following hypothetical scheme of determination of coat color in a mammal. Gene A controls the conversion of a white pigment P 0 into a gray pigment P 1 ;
If you obtain an RF value of 45 percent in one experiment, what can you say about linkage? (The actual figures are 58, 52, 47, and 43 out of 200 progeny)
Why the parents wake up, other than the children did didn't wake up. A gene for sweat gland production is found on the X chromosome.
The termination of translation occurs when a ribosome that translating an mRNA encounters a stop codon. Since there are no tRNAs that can base pair with stop codons.
In an experiment you are studying a neuron that receives synaptic inputs from two other neurons: one from neuron A and one from neuron B. Illustrate a separate graph for each value of Vm.
In wild petunias, the allele for red flower color ( R ) is dominant over the allele for white flower color ( r ). We sample a population and find 225 plants have white flowers and seventy-five have red flowers.
Disease and treatment associated with the neurotransmitter imbalance. Describe the significance of the dopamine neurotransmitter in our body.
Dr. Ara B. Dopsis and Dr. C. Ellie Gans are performing genetic crosses on daisy plants. They self-fertilize a blueflowered daisy and grow one hundred progeny plants that consist of 55 blue-flowered plants,
Recently, use of cleaner fuels has greatly reduced soot in the landscape, and dark-coloured moths have been disappearing. Should the two forms of moths be considered separate species.
Assume you use phase contrast microscopy to observe a wet mount of a soil sample and a goo sample. In what ways are potential ET microbes similar to microbes previously characterized on earth?
Give 2 distinguishing characteristics of a tRNA molecule. In translation what does the ribosome do? With reference to translation, what does translocation refer to?
Does this represent a significant section from the Hardy-Weinberg ratios? Further studies show that the absence of the A/A genotype is due to the lethal effect of the A allele when homozygous.
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd