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Question- You digested a protein with trypsin, chymotrypsin, and CNbr. For each of the proteolytic fragments were then sequenced and the data is shown below. from this data, list the amino acids in the correct order to get the sequence of the undigested protein.
Trypsin digest: T-Y-R M-L-F-G-M-K H-E
Chymotrypsin digest; R-H-E M-L-F G-M-K-T-Y
CNBr digest: L-F-G-M M K-T-Y-R-H-E
Express your answers to three significant figures separated by comma.
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An automobile tire has a pressure of 255 kPa gauge in the summer when the tire temp after driving is 50C. Explain what is the winter time pressure of the tire at 0C if the volume of the tire is considered the same and there are no leaks
In the purification process, the organic layer is washed with 2 M NaOH and then with water. What is the purpose of these washes
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