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You are investigating 4 recessive alleles in corn. They are whimpy ears (w), striped leaves (s), shriveled kernels (k), and red tassels (r). Their dominant counterparts are represented by the "+" symbol. A plant that is homozygous recessive for all four genes (ww ss kk rr) is crossed with a plant that is homozygous dominant for all four genes (++ ++ ++ ++). The F1 progeny (w+ s+ k+ r+) are then testcrossed with another plant. A total of 1000 F2 progeny were produced. The F2 progeny display the following phenotypes.
Phenotype
Observed # of Progeny
w s k r
198
+ + + +
202
w s k +
201
+ + + r
199
w + k +
49
+ s + r
51
w + k r
48
+ s + +
52
a. Calculate the recombination frequencies between each pair of genes. (HINT: you will calculate 6 recombination frequencies - determine where each pair of genes experienced a crossover to produce the observed offspring).
b. Draw a chromosome map for these genes if they are linked.
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