Calculate the mg of phosphate in each ml of specimen

Reference no: EM13711209

A 2.00mL specimen was treated with reagents to generate color with phosphate following this, the sample was diluted to 100 mL. Photometric measurement for the phosphate in a 25mL aliquot showed an absorbance of 0.428. Addition of 1mL of a solution containing 0.500mg phosphate to a second 25 mL aliquot yielded an absorbance of 0.517. Calculate the mg of phosphate in each mL of specimen.

Give your answer with short explanation

Reference no: EM13711209

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