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5.00-mL solution aliquots contain PCP were carefully measured into five separate 50.00-mL volumetric flasks and made basic with KOH. The following volumes of a standard solution of PCP (2.000 µg/mL) were added into each flask: 0.000, 0.500, 1.00, 1.50 and 2.00-mL the fluorimeter readings from each solution are listed below. Calculate the concentration of PCP in the sample.
spike VOl. (mL)
spike amount (µg)
spike conc.(µg/mL)
readout
0.00
0.000
0.0000
3.26
0.500
1.000
0.02000
4.8
2.000
0.04000
6.41
1.500
3.000
0.06000
8.02
4.000
0.08000
9.56
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