Single stranded conformation polymorphism analysis, Biology

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 

Posted Date: 3/6/2013 6:03:56 AM | Location : United States







Related Discussions:- Single stranded conformation polymorphism analysis, Assignment Help, Ask Question on Single stranded conformation polymorphism analysis, Get Answer, Expert's Help, Single stranded conformation polymorphism analysis Discussions

Write discussion on Single stranded conformation polymorphism analysis
Your posts are moderated
Related Questions
explain in detail the cell theory and it exceptions


#question. what is cloning.

Define Vacuum production and Thermal processing - Canning? Vacuum production - This can be obtained by filling the heated product into the can, by heating the can and content

Define important aspects of nutritional needs during emergency situations? This unit discussed important aspects of nutritional needs during emergency situations and under envi

Which of the following characteristics is not shared by typical birds and mammals? A) Four chambered heart B) Milk production C) Four limbs D) Endothermy E) Insulating skin structu

Informatics  is the study of the application of computer and statistical techniques to the management of information. In the genome projects, informatics includes the development o

Define the Utilization of Most Probable Number Techniques? The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The

Stage two using a mucoperiosteal flap A Mid crestal incision over the implant site is made and reflection of mucoperisteal flap is done under local anesthesia. After locating t

TYPE S -   There are present three types of muscle fibres:   1.      Striped or striated or skeletal or voluntary muscles.   2.      Unstriped or non-striated o