Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.
This technique includes 4 steps
1. Digestion of genomic dna through restriction endonucleases
2. Denaturation in alkaline solution.
3. Electrophoresis on neutral polyacrylamide gel.
4. Transfer it to a nylon membrane.
5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.
6. dhplc
Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.
Define early ebb or shock phase - Physiological Response to injury? This is usually brief in duration lasting 12 to 24 hours and occurs immediately following injury. Blood pres
Explain the Bioavailability of nutrients? The amount of many nutrients needed in the diet depends on the absorption or bioavailability of the nutrients. Bioavailability is defi
Q. Can you explain Ventricular Septal Defect? VSD can be found in various part of ventricular septum hence all possible views except suprasternal views are used to detect an
Q. Define Diabetic Ketoacidosis? Diabetic Ketoacidosis (DKA) is one of the acute complications of diabetes mellitus. The name itself implies that there is acidosis (decrease in
Phylum Zygomycetes 1) Asexual reproduction by non-motile spores produced from a stalked spore case which is known as sporangium. 2) They reproduced by- conjugation between n
C o mbination of meat products Combining meats and byproducts of different species to produce value added products results in complementation and supplementation of their qua
Explain the Physiology of Lactation? Lactogenesis is the onset of copious milk secretion around parturition, triggered by a fall in plasma progesterone levels. Although some co
Properties of Cardiac Cells Automatically: ability of the heart to initiate impulses regularly and spontaneously. Excitability: ability of cardiac cells to respond to
(i) Name one fibrous and one globular protein. fibrous globular (ii) Outline the difference in structure between a fibrous and a globular protein. ' shows the ef
I need the Answers for the final exam for biology it''s a one hundred question test if you can help i would appreciate it
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd