Reciprocal translocation and fusion, Biology

In chronic myelogenous leukemia, white blood cells proliferate ceaselessly. In affected white blood cells, the BCR-ABL oncogene, the result of a gene fusion, transmits a constitutive growth signal. The severity of the disease correlates with the fraction of white blood cells that carry the BCR-ABL oncogene.

a) How does reciprocal translocation and fusion of the BCR and ABL genes lead to unregulated cell dIvision and leukemia

One treatment for the chronic myelogenous leukemia is a drug that inhibits the BCR-ABL protein and prevents it from transmitting the growth signal. This can result in remission of the disease by preventing proliferation of the cancer cells. During treatment of the disease, the effectiveness of treatment is monitored by assessing the level of the BCR-ABL oncogene present in a fixed quantity of white blood cells. Suppose that the structure of the DNA in the nonmutant BCR and ABL genes are as shown here, and that of the BCR-ABL oncogene is as depicted.

Short oligonucleotide primers that bind to the BCR and ABL genes as shown (the 3' end of each primers is at the tip of the arrow) are labeled 1, 2, 3, and 4.

553_Reciprocal translocation and fusion.png

b) How would you detect the presence of the BCR-ABL oncogene ?

c) If the drug is working, what would you expect to see ?

Posted Date: 2/18/2013 2:40:40 AM | Location : United States







Related Discussions:- Reciprocal translocation and fusion, Assignment Help, Ask Question on Reciprocal translocation and fusion, Get Answer, Expert's Help, Reciprocal translocation and fusion Discussions

Write discussion on Reciprocal translocation and fusion
Your posts are moderated
Related Questions
Absorption The monosaccharides, amino acids and other products of digestion must be passed on to other tissues to be useful for the organism. The process by which the digested

The law of contract is that the branch of law which determines the circumstances in which promises made by the parities to a contract shall be legally binding on them. Its rules de

Coding sequence is the portion of the gene or the mRNA which actually codes for a protein. Introns are not coding for the sequences; nor are the 5' or 3' untranslated regions (or

Q. What are the allosteric enzymes? Allosteric enzymes are those that have in excess of one activation center and to which other substances, called allosteric regulators, bind.

The protein truncation test (ptt) gives an uncommon exception, targeting mutations that create shortened proteins, majorly premature translation termination. Ptt has various attrac

Is the transpiration the only way through which leaves lose water? The Plants don't only lose water as vapor as by transpiration. Leaves also lose liquid water by a phenomenon

Larval forms - Porifera In the majority of sponges the larval stage develops in the body of the parent. The larva is generally at the blastula stage of development. Figure dis

Before we further proceed to discuss the fossil history of the humans we shall briefly look into what distinguishes humans or genus Homo from his ancestors namely the apes. These d

Describe what happens during activation of the lac operon. Lactose binds to the repressor protein, which causes the repressor protein to be released from the operator site. Th

Internal Mammary Artery (1MA) :  This is an excellent graft. When left internal mammary artery (LIMA) is anastomosed to LAD, which has more than 70 per cent block, patency at