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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Q. What is the difference between disaccharides and monosaccharides? What are some examples of monosaccharides and of disaccharides that form them? Monosaccharides are simple m
(a) What is the distinction among 'breathing' and 'respiration'? (b) What part do 'ventilation' and 'gaseous exchange' play in these processes? (a) Breathing i
Describe 5 ways that a microorganisms directly or indirectly affects our lives.
Q. What is the importance of iron in diet? What is the disease caused by iron deficiency? Iron acts as a constituent of the hemoglobin molecule and of enzymes of the energetic
Explain Chlamydia A single 1 g dose of azithromycin (Zithromax) or 7 days' treatment with a tetracycline are both effective for treatment of uncomplicated urethral or cervical
Theories are import because they can be used to a. Test research hypothesis b. Develop questions which will facilitate more effective research c. Conduct more meaningful and useful
Low levels of dissolved salts characterise the freshwater biomes. The salt content of fresh water is about 0.005 percent. The freshwater biomes consist of inland bodies of standing
Enzyme glutamate oxaloacetate transaminase catalyzes the reaction between glutamate and oxaloacetate with the formation of a-ketoglutarate and aspartate due to transfer of one
In the eukaryotes, eukaryotic release factor eRF-1 recognizes all three termination codons that are UGA UAA, and UAG and, with the help of protein eRF-3, terminates translation.
Determine number of sucrose molecules in the solution When a solid is dissolved in a volatile solvent the vapour pressure of the solution is less than the vapour pressure of th
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