Protein truncation test, Biology

The protein truncation test (ptt) gives an uncommon exception, targeting mutations that create shortened proteins, majorly premature translation termination. Ptt has various attractive features, involving pinpointing the site of a mutation, a low false-positive rate, good sensitivity, and, more esssentially, the near-exclusive highlighting of disease-causing mutations. Additionally, ptt initiated the detection of a new mutation type, that is, a sequence change creating a hypermutable region surfacing in rna. This technique takes rna as template and scans for mutations at protein level.

Protein truncation test includes four steps

1. Isolation of nucleic acid, that is, total rna, genomic dna or poly (a) rna.

2. Amplification of particular gene of interest

3. In vitro translation and transcription of the product of amplification reaction

4. Identification of translation of the products through autoradiography.

The shorter protein products of the mutant alleles are simply distinguished from the full length protein of mutant allele.

 

 

Posted Date: 3/6/2013 6:02:59 AM | Location : United States







Related Discussions:- Protein truncation test, Assignment Help, Ask Question on Protein truncation test, Get Answer, Expert's Help, Protein truncation test Discussions

Write discussion on Protein truncation test
Your posts are moderated
Related Questions
Q. Definition of Counselling? Counselling has been defined as a process of helping/enabling a person/people solve certain interpersonal (not able to continue relationship), emo

Pr o d u c t i o n Diseases The production diseases, which were previously referred as metabolic diseases, include diseases like parturient paresis (milk fever), downer

ENDOCRINE GLANDS OF AN INSECT - The endocrine glands of cockroach are as follows. 1.       Intercerebral gland cells. These cells lie in the brain. They secrete brain horm

Q. Which is the kind of muscle tissue that performs the peristaltic movements of the intestines? The smooth muscle tissue is responsible for the peristaltic movements of the in

Define cooking,blanching and Canning - thermal processing? Cooking: Cooking is a primary process to make food more palatable and improve taste. This is not used as a preservati

How Vaginal infections cause Sulfonamide creams, other ''broad-spectrum'' vaginal preparations, and currently available preparations of Lactobacillus  species or dairy products

Explain the Structure of Fungi? The vegetative structure of a fungus is called thallus. It varies in complexity and size, ranging from unicellular yeasts to multicellular mould

Q. Limitations in the Diagnostic use of Stress Echocardiography? Although the presence of coronary artery disease is readily recognised in the setting of multivessel disease, a

A haploid cell contains: A.one half of a complete set of chromosomes B.several complete sets of chromosomes C.the correct number of chromosomes D.two complete sets of chromosomes E

Endocrine Interactions At the beginning of menstruation the inhibitory influence of the corpus luteum on the pituitary is removed and FSH is secreted in increasing amounts. Th