Protein truncation test, Biology

The protein truncation test (ptt) gives an uncommon exception, targeting mutations that create shortened proteins, majorly premature translation termination. Ptt has various attractive features, involving pinpointing the site of a mutation, a low false-positive rate, good sensitivity, and, more esssentially, the near-exclusive highlighting of disease-causing mutations. Additionally, ptt initiated the detection of a new mutation type, that is, a sequence change creating a hypermutable region surfacing in rna. This technique takes rna as template and scans for mutations at protein level.

Protein truncation test includes four steps

1. Isolation of nucleic acid, that is, total rna, genomic dna or poly (a) rna.

2. Amplification of particular gene of interest

3. In vitro translation and transcription of the product of amplification reaction

4. Identification of translation of the products through autoradiography.

The shorter protein products of the mutant alleles are simply distinguished from the full length protein of mutant allele.

 

 

Posted Date: 3/6/2013 6:02:59 AM | Location : United States







Related Discussions:- Protein truncation test, Assignment Help, Ask Question on Protein truncation test, Get Answer, Expert's Help, Protein truncation test Discussions

Write discussion on Protein truncation test
Your posts are moderated
Related Questions
Define Nutrition and Sports - Physical Fitness of an athlete? The physical fitness and performance of an athlete is affected by both current and previous nutritional status. T

write a comprehensive note on vitamins?

Q. Effects of smoking on oesophagitis? 1. A decrease in pyloric sphincter pressure which may predispose an individual to duodenal ulcer. 2. An alteration in the nature of ga

What are colonies and societies? Colonies are functional integrated aggregates produced by individuals of the same species. Colonies are often confused with a one individual. E

what is the respiratory organ of phylum protozoa?

What do digestive enzymes do to food? Digestive enzymes suspend food; make food soluble, break large insoluble food molecules into smaller, soluble molecules

Leptospirosis Synonyms: Weil’ disease, canicola fever, 7-day fever Leptospirosis is an important and widespread zoonosis. It was first described by Adolf Weil in 1886 when h

Animal studies Most toxicological data for risk assessment are derived from the animal studies and  it  is, therefore, essential that these studies be conducted following widel

C a nned and Retort Pouched Meat Products Canning as a process contributes to preservation, storage and distribution of food supplies. Production of thermally processed meat

Assuming that the equilibrium potential for sodium ion across a skeletal muscle is +66 mv at a temperature of 37 degree celcius when the external concentration is 145 mM and the in