Procedures for diagnosis - isolation of etiological agent, Biology

Isolation and identification of the etiological agent:

Isolation of the etiological agent is attempted in chicken embryos, cell cultures, laboratory animals, culture media or the host species. Cell cultures are most ideal, economic and often give quick results for virus agent. These could be homologous or heterologous; primary, secondary or established cell lines, and of diverse tissue origin (kidney, testes, liver, lung, whole embryo, etc.) depending upon the virus under study. If the viral agent involved is not known, primary kidney or thyroid cell cultures from embryo or young animals of the same (homologous) species should be preferred. The inoculums, after making it free from bacterial and fungal contamination by suitable antibiotic/antifungal treatment, are given 3 to 5 blind passages in a particular cell culture.

Type of cytopathic effects (CPE), demonstration of virus particles/ antigen in the infected cells, haemadsorption, haemagglutination (HA), haemagglutination inhibition (HI), neutralization of CPE with specific serum and physiochemical and biological properties may be studied for identification of the virus. When cell culture facilities are not available or the virus does not grow in any cell culture or produce CPE, chicken embryos may be inoculated in appropriate route i.e. CAM, AC, YS, amniotic cavity or intravenous. Laboratory animals viz. mice, guinea pig, hamster, rabbit, chicken, etc., which support the growth of a number of pathogens may also be used. Route of inoculation for these animals are selected in accordance with the agent being looked for. Strictly host specific viruses those do not grow in any cell culture or chicken embryos, the host species has to be used for its isolation.

Posted Date: 9/19/2012 1:18:58 AM | Location : United States







Related Discussions:- Procedures for diagnosis - isolation of etiological agent, Assignment Help, Ask Question on Procedures for diagnosis - isolation of etiological agent, Get Answer, Expert's Help, Procedures for diagnosis - isolation of etiological agent Discussions

Write discussion on Procedures for diagnosis - isolation of etiological agent
Your posts are moderated
Related Questions
Respiratory System - Developmental Changes after Birth The new-born's lungs are collapsed and need powerful breaths to inflate them; airways too are small, offering remarkable

Briefly explain about the Flexed-arm Hang Test? Persons who cannot do one pull-up may do the flexed-arm hang. Using the same hand position as in pull-ups, subject takes flexed-

Q. What is an example of intraspecific competition? The Intraspecific competition occurs in practically all species, for instance, the competition of humans for a job.

Where is the gall bladder located? The gall bladder is located in the Upper Right Quadrant (URQ) of the abdomen just below the liver. It keeps bile secreted by the liver. The d

Take the prepared slide of Euglena and focus it first under low power and next under the high power of tlie microscope. Observe tlie following characteristics: i) Euglena is an

Define Descending type of paper chromatography? In descending chromatography, the movement or flow of mobile phase is in downward direction. In this technique the solvent is ke

There are several anaplerotic reactions required to replenish intermediates of the TCA cycle. All of the following compounds can be used for these anaplerotic reaction except: -

tertiary structure of protein


Q. What is the difference between the concepts of epidemic disease and endemic disease? The Endemic diseases are those that often affect people of a given place, many or few in