Green fluorescent protein - mitochondrial inner membrane, Biology

You are studying how proteins are targeted to the mitochondria. MIP1 is in the mitochondrial inner membrane, whereas MIP2 is targeted to the matrix. You are most interested in how proteins get into the inner membrane, so you focus on MIP1.

A) Analysis of a GFP-tagged MIP1 in a Tom 20 yeast mutant suggests that MIP1 targeting is Tom 20-dependent. Where would the GFP-tagged MIP1 be found in this mutant? Include your rationale in your answer.

B) Another scientist has already identified the matrix-targeting sequence at the N-terminus of MIP1. You think you have also found a stop-transfer sequence. How would you determine if the sequence you identified is NECESSARY for targeting to the membrane? You have the following tools (and you don't have to use all of them):

-GFP-tagged MIP1

-GFP-tagged MIP1 with the putative stop-transfer sequence mutated

-GFP-tagged MIP2

-GFP-tagged MIP2 with the putative stop-transfer sequence added


Describe the experiment including controls. State your predicted results if the sequence is necessary for inner membrane targeting.

Posted Date: 3/6/2013 5:42:50 AM | Location : United States

Related Discussions:- Green fluorescent protein - mitochondrial inner membrane, Assignment Help, Ask Question on Green fluorescent protein - mitochondrial inner membrane, Get Answer, Expert's Help, Green fluorescent protein - mitochondrial inner membrane Discussions

Write discussion on Green fluorescent protein - mitochondrial inner membrane
Your posts are moderated
Related Questions
Fuel Requirements - Deforestation The increasing demand for fuel wood is one of the major factors leading to the degradation of the forest ecosystem. Fuel wood is of such majo

Biotic Balance of Nature A nineteenth century Biologist is reported to have expressed, Glory of Britain is due to its old maids He reasoned Healthy Britons are nourished by roa

Q. What is the major evolutionary innovation presented by nematodes? What is the benefits of that innovation? The major evolutionary innovation of nematodes is the complete dig

In earthworms, when the ovaries mature, the clitellum secretes a viscous substance in the form of a girdle. This girdle hardens in to a cocoon around the clitellum. The ova are dis

You have a neuron with a resting potential of -55 mV, EK of -70 mV, and ENa of 50 mV. You decide to voltage clamp the neuron. Now, draw the membrane current over time. What will th

The Red Data Book Species judged as threatened are listed by various agencies as well as by some private organisations. The most cited of these lists is the Red Data Book. It

what the by products of sugarcane

Pathogenesis The mode of intracellular replication of chlamydial agents is investigated morphologically and cytochemically with both light and electron microscope. It is estab

Explain Procedure for Simple Staining of Bacterial Cultures? Now carry out the exercise following the steps given herewith: (1) Prepare thin bacterial smear on clean glass s

How is the blood typing concerning the ABO system and the Rh usually done? In the blood typing for the ABO system and the Rh system a blood sample is collected from the person