Explain procedure for preparation of general purpose media, Biology

Assignment Help:

Explain the Procedure for Preparation of General Purpose Media

Procedure: Now prepare the media following the steps enumerated herewith:

1. Weigh and dissolve each ingredient except agar in 500 ml of distilled water.

2. Make the volume to 1000 ml with distilled water. Set the pH to 7.0. This is a nutrient broth.

3. Autoclave the nutrient broth and dispense in sterile test tubes aseptically.

4. For nutrient agar, add 15 gms of agar in 1000 ml of nutrient broth. Heat the mixture to dissolve agar.

5. Dispense the mixture into flasks and autoclave at 15 psi for 15-20 minutes.

6. After autoclaving, cool the nutrient agar medium to 45°C and dispense it aseptically into sterile petri plates and tubes to make agar plates, slants and deep tubes.

7. Allow to solidify. For making slants, keep the tubes in inclined position for agar solidification.

8. Inoculate nutrient agar plates and tubes and nutrient broth with bacterial culture and incubate for 24 hours.

9. Note the observations and results.


Related Discussions:- Explain procedure for preparation of general purpose media

Wace, Excretory organ of lizard.

Excretory organ of lizard.

What do you mean by amphibians of the plant world, Q. Why can the bryophyte...

Q. Why can the bryophytes be considered the "amphibians of the plant world"? Like adult amphibians, the bryophytes live in the terrestrial environment but they depend on water

The prs kit for post removal uses-endodontics principles, The PRS kit is co...

The PRS kit is comprised of          -5 variously sized trephines and corresponding tubes according to material if it's silver or silver point          -A transmetal bur

Define briefly about the nutrition security, Define Briefly about the Nutri...

Define Briefly about the Nutrition security? Nutrition security can be briefly defined as a balance between biological requirements in energy and nutrients and the quantity and

Explain what is genetic engineering, Explain brifly what is Genetic Enginee...

Explain brifly what is Genetic Engineering ? Genetic Engineering : Engineering techniques have been used in agriculture and horticulture for centuries. Certain plants or ani

Transplantation, Transplantation - It is implanting of a living or p...

Transplantation - It is implanting of a living or preserved tissue or organ from one site to another side in the same individual or from one individual called donor to recip

Why is the cartilage more flexible than bone, why is the cartilage more fle...

why is the cartilage more flexible than bone? in general, why does cartilage take longer to repair than bone?

Vertebrate limb, Vertebrate Limb Earlier we know that how a series of...

Vertebrate Limb Earlier we know that how a series of sequential and coordinated interactions between different cell groups and tissues carry about the construction of a compl

Poultry and duck diseases-avian reovirus infections, Avian reovirus infecti...

Avian reovirus infections There are several avian reoviuses isolated from chicken, turkey and other species of birds. They are associated with arthritis (tenosynovitis), respir

Nutrition, define alimentary canal

define alimentary canal

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd