Explain Direct Microscopic Count - Measurement of Microbes?

The most obvious way to determine microbial number is through direct counting under a microscope. In this method, a measured volume of the sample is placed within defined area on the microscopic slide and cells are counted under the microscope. In the breed count method for milk, 0.1 ml of the sample, is spread over a marked square centimeter area on a slide. Stain is added and the microbes are counted under a microscope for several different viewing fields. The average number of microbes/field can be calculated and used for calculating the number of microbes per square centimeter area or per 0.1 ml of the sample. Specially designed counting chambers like Petroff Hausser counting chamber, haemocytometer and electronic counter like coulter counter can also be used for direct measurement.

Posted Date: 6/7/2013 1:03:55 AM | Location : United States

Ask an Expert

Related Discussions:- Explain direct microscopic count - measurement of microbes, Assignment Help, Ask Question on Explain direct microscopic count - measurement of microbes, Get Answer, Expert's Help, Explain direct microscopic count - measurement of microbes Discussions

Write discussion on Explain direct microscopic count - measurement of microbes
Your posts are moderated

Write your message here..

Related Questions

Invertebrates, 1) What are some characteristics that define the lifestyle o... 1) What are some characteristics that define the lifestyle of a parasitic nematode? 2) What are beneficial nematodes? Discuss their significance

Common features of eye and limb development, Common Features of Eye and Lim... Common Features of Eye and Limb Development A comparison of the embryonic development of the very much different organs, the eyes and the limb, points to various interesting

Define proteolytic enzymes, Proteolytic enzymes They catalyze the hydro... Proteolytic enzymes They catalyze the hydrolysis of peptide bonds in other proteins. In order to prevent them doing generalized damage to all cellular proteins, they are often

Explain horizontal full mucoperiosteal flaps, Explain Horizontal Full Mucop... Explain Horizontal Full Mucoperiosteal Flaps - Endodontic Surgery      a) Also called:  Envelope flap, b) Only a Horizontal incision, without the vertical releasing incision

Precautions for spore staining in a given bacterial culture, Define some Pr... Define some Precautions for spore staining in a given bacterial culture? 1. Do not allow malachite green to dry on slide. 2. Decolourization should be done only after coolin

How much calories in dried fruits, How much calories in Dried fruits Dr... How much calories in Dried fruits Dried fruits are exceptionally rich in calories and they supply significant amounts of minerals. Of the vitamins found in fruits, β-Carotene a

Determine the general principles for incisions, General Principles for Inci... General Principles for Incisions The general principles for placing incisions so as to create surgical flaps remain the same regardless of the reason for creating them. These a

Why does the ingestion of alcohol increase diuresis, Q. Why does the ingest... Q. Why does the ingestion of alcohol increase diuresis? Alcohol inhibits the secretion of ADH that is antidiuretic hormone by the pituitary that is why when it is drunk to exce

Explain shear pin model, Q. Explain Shear Pin Model? Shear Pin Model: I... Q. Explain Shear Pin Model? Shear Pin Model: In this type, a shearing force is applied using a pin to shear the food Product. We are rupturing the element of the food product b

What is photosynthesis , What is Photosynthesis ? Photosynthesis is t... What is Photosynthesis ? Photosynthesis is the method by which plants trap radiant energy from the sun and convert the energy into a biochemical form. This biochemical energy