Explain Direct Microscopic Count - Measurement of Microbes?

The most obvious way to determine microbial number is through direct counting under a microscope. In this method, a measured volume of the sample is placed within defined area on the microscopic slide and cells are counted under the microscope. In the breed count method for milk, 0.1 ml of the sample, is spread over a marked square centimeter area on a slide. Stain is added and the microbes are counted under a microscope for several different viewing fields. The average number of microbes/field can be calculated and used for calculating the number of microbes per square centimeter area or per 0.1 ml of the sample. Specially designed counting chambers like Petroff Hausser counting chamber, haemocytometer and electronic counter like coulter counter can also be used for direct measurement.

Posted Date: 6/7/2013 1:03:55 AM | Location : United States

Ask an Expert

Related Discussions:- Explain direct microscopic count - measurement of microbes, Assignment Help, Ask Question on Explain direct microscopic count - measurement of microbes, Get Answer, Expert's Help, Explain direct microscopic count - measurement of microbes Discussions

Write discussion on Explain direct microscopic count - measurement of microbes
Your posts are moderated

Write your message here..

Related Questions

Explain the treatment of iron deficiency anaemia, Explain the Treatment of ... Explain the Treatment of iron deficiency anaemia? Oral iron is the preferred method of treatment of IDA. The dosage is decided depending on the severity of the condition. Gener

Drawbacks of nutritional status that assessed traditionally, Define drawbac... Define drawbacks of Nutritional status that assessed traditionally? Nutritional status assessed traditionally with the use of anthropometric, biochemical clinical and dietary a

Define starch - carbohydrates, Define Starch - Carbohydrates? Starch is... Define Starch - Carbohydrates? Starch is the major form of stored carbohydrate in plants. Starch, as you may already know, is composed of a mixture of two substances: amylose,

What is combination therapy, What is Combination therapy Combination th... What is Combination therapy Combination therapy - Use of combination therapy for treatment of immunosuppressed patients with invasive aspergillosis (IA), which has a high morbi

Maintenance and regulation of peripheral circulation, Maintenance and Regul... Maintenance and Regulation of Peripheral Circulation Pressure receptors (baroreceptors) located in the wall of the internal carotid arteries and in the arch of aorta, when

Weaning the infant from the incubator, Weaning the Infant from the Incuba... Weaning the Infant from the Incubator When heater output reading is minimal or nil it suggest that infant is capable of generating enough metabolic heat to keep himself war

Give a specific example for epithelial, PROVIDE a specific example for epit... PROVIDE a specific example for epithelial and connective tissues, how the arrangement of cells helps with tissue functioning?

Pour plate method, what is advantages and disadvantages of the pour plate m... what is advantages and disadvantages of the pour plate method

How mechanical soft diet is a normal diet, How mechanical soft diet is a no... How mechanical soft diet is a normal diet The mechanical soft diet is a normal diet that is modified only in texture for ease of mastication. This is used when a patient canno

Incineration treatment technologies for health-care waste, Q. Incineration ... Q. Incineration Treatment technologies for health-care waste? It used to be the method of choice for most hazardous health-care wastes and is still widely used. It is a high te